Language
LMH鸡肝癌细胞系 高性价比
培养基：90%高糖DMEM+10%FBS
传代方法：Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution.
Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
LMH鸡肝癌细胞系 高性价比
生长条件：95%空气+5%二氧化碳 37℃
生长特性：贴壁生长
存储条件：50%高糖DMEM+40%FBS+10%DMSO 液氮
Strain：Leghorn
Karyotype：triploid; modal number = 116; six marker chromosomes
Derivation：LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan.
Clinical Data：Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
Receptor Expression： estrogen receptor, positive
Tumorigenic ：Yes
Effects：Yes, forms tumors in nude mice
Year of Origin ：1981
LMH鸡肝癌细胞系 高性价比
描述 ：Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
The morphology of the cells is dendritic like.
The cells express glucose-6-phosphatase and weak canalicular ATPase activity.
The cells form tumors in athymic nude mice.
LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene.
The cell line is useful for transfection studies.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.
Vessels prepared in this fashion may be used immediately.
LMH鸡肝癌细胞系 高性价比
