Language
23714
Product format:
Freeze-dried
Strain designation:
CH 19299 [NCTC 10112]
Deposited as:
Mycoplasma orale Taylor-Robinson et al.
Type strain:
Yes
Mycoplasma orale strain CH 19299 [NCTC 10112] is a whole-genome sequenced bacterial type strain isolated in Washington, DC, from the oropharynx of a human child. This product has applications media testing and quality control.
Handling procedureFollow instructions as suggested for the culturing of Mollicutes:
PROCEDURES FOR PROPAGATING MOLLICUTES:
Open the vial according to the enclosed instructions.Using a Pasteur or 1.0 mL pipette, withdraw approximately 0.5 to 1.0 mL from a tube containing 2.25 mL. Rehydrate the entire pellet. Aseptically transfer this aliquot back into the tube. Mix well.Make serial dilutions by transferring 0.25 mL from the original tube to a tube containing 2.5 mL. Repeat process by transferring 0.25 mL from the second to a third tube, etc. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze drying process often inhibits growth.Plates may be inoculated to check colony morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony forming units. However, not all strains do well on solid medium.Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation. Depending on the medium used, growth will be indicated by increased turbidity, a color change,or both.
Tubes and plates are incubated under anaerobic conditions using an anaerobe jar or other appropriate method. The incubation temperature is 37°C.Medium to light turbidity will appear in the first few dilution tubes within 72 hour to 7 days. Colonies generally appear on solid medium within 7 days.Subsequent, fresh transfer will grow more rapidly than the original rehydrated culture; but usually still requires 48 hours between transfers.Handling notesPurified genomic DNA of this strain is available as ATCC® 23714D™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
以下为部分产品目录：
白色念珠菌 ATCC 90029
肺炎克雷伯菌ATCC 700603
大肠艾希氏菌ATCC 35218
表皮葡萄球菌ATCC 12228
金黄葡萄球菌ATCC 29213
金黄葡萄球菌ATCC 25923
偶然分支杆菌ATCC 6841
嗜热脂肪杆菌ATCC 7953
嗜热脂肪芽孢菌ATCC 10149
流感嗜血杆菌ATCC 10211
副氏志贺氏菌ATCC 12022
堪萨斯分枝杆菌ATCC 12478
脑膜炎奈瑟菌ATCC 13090
产气夹膜梭菌ATCC 13124
胞内分枝杆菌ATCC 13950
伤沙门氏菌ATCC 14028
溶藻弧菌 ATCC 17749
副溶血性弧菌ATCC 17802
单核增生李氏特菌ATCC 19114
化脓性链球菌ATCC 19615
结合分枝杆菌ATCC 25177
脆弱拟杆菌 ATCC 25285
坏死梭杆菌 ATCC 25286
具核分枝杆菌ATCC 25586
大肠埃希氏菌ATCC 25922
金黄葡萄球菌ATCC 25923
宋氏志贺氏菌ATCC 25931
厌氧消化链球菌ATCC 27337
粪肠球菌 ATCC 29212
空肠弯曲杆菌ATCC 29428
英诺克李斯特菌ATCC 33090
大肠杆菌O157ATCC 35150
阪崎肠杆菌ATCC 51329
蜡样芽孢杆菌ATCC 11778
白色假丝酵母/白色念球菌 ATCC 10231
鲜绿青霉 ATCC 10515
桔青霉 ATCC 1109
蜡样芽孢杆菌 ATCC 11778
福氏志贺氏菌(Shigella flexneri) ATCC 12022
表皮葡萄球菌 ATCC 12228
败毒梭菌Clostridium septicum ATCC 12464
阴沟肠杆菌 ATCC 13047
产气荚膜梭菌 ATCC 13124
荧光假单胞菌 ATCC 13525
婴儿双歧杆菌 ATCC 15697
短双歧杆菌 ATCC 15700
青春双歧杆菌 ATCC 15703
恶臭假单胞菌 ATCC 17485
单增李斯特氏菌 ATCC 19111
溶组织梭菌Clostridium histolyticum ATCC 19401
生孢梭菌 ATCC 19404
破伤风杆菌/梭菌Clostridium tetani ATCC 19406
化脓性链球菌 ATCC 19615
黄杆菌属 ATCC 21047
近平滑念珠菌 ATCC 22019
变异链球菌 ATCC 25175
脆弱拟杆菌 ATCC 25285
洋葱假单胞菌 ATCC 25416
大肠埃希氏菌 ATCC 25922
宋内氏志贺氏菌(Sh.sonnei) ATCC 25931
创伤弧菌 ATCC 27562
铜绿假单胞杆菌（绿脓杆菌） ATCC 27853
粪肠球菌 ATCC 29212
两歧双歧杆菌 ATCC 29521
多形拟杆菌 ATCC 29741
粪肠球菌 ATCC 33186
空肠弯曲菌 ATCC 33291
金黄葡萄球菌 ATCC 33591
大肠埃希氏菌 ATCC 35218
屎肠球菌 ATCC 35667
迟缓真杆菌/迟缓埃格特菌 ATCC 43055
弗氏柠檬酸杆菌 ATCC 43864
淋病柰瑟氏菌 ATCC 49226
流感嗜血杆菌 ATCC 49247
肺炎链球菌 ATCC 49619
流感嗜血杆菌 ATCC 49766
粪肠球菌(高耐) ATCC 51299
阪崎肠杆菌 ATCC 51329
克柔念珠菌 ATCC 6258
枯草芽孢杆菌 ATCC 6633
肺炎克雷伯菌 ATCC 700603
热带念珠菌 ATCC 750
嗜热脂肪地芽孢杆菌 ATCC 7953
植物乳杆菌 ATCC 8014
大肠埃希氏菌 ATCC 8739
白色假丝酵母/白色念球菌 ATCC 90029
铜绿假单胞杆菌（绿脓杆菌） ATCC 9027
枯草芽孢杆菌黑色变种 ATCC 9372
艰难梭菌Clostridium difficile ATCC 9689
啤酒酵母 ATCC 9763
砖红链霉菌 ATCC19776
白色葡萄球菌 ATCC8799
皱褶青霉 ATCC 10128
酿酒酵母 ATCC 26603
酿酒酵母 ATCC 26785
运动发酵单孢菌 ATCC 29191
假单胞杆菌 ATCC 31554
粪产碱杆菌 ATCC 31555
菅囊酵母 ATCC 32691
弗氏柠檬酸杆菌 ATCC10787
荚膜红细菌 ATCC11166
发根土壤杆菌（发根植物单胞菌） ATCC11325
生孢梭菌（产芽孢梭状芽孢杆菌） ATCC11437
地衣芽孢杆菌 ATCC11946
谷氨酸棒杆菌 ATCC13032
熏衣草灰链霉菌 ATCC13306
猪霍乱沙门氏菌猪霍乱亚种 ATCC13312
脱氮假单胞菌 ATCC13867
耐放射异常球菌 ATCC13939
绿针假单胞菌 ATCC13985
肠门氏菌肠炎亚种 ATCC14028
巨大芽孢杆菌 ATCC14581
吸水链霉菌奥萨霉素亚种 ATCC15420
长双歧杆菌 ATCC15707
放射性根瘤菌 ATCC15955
短密青霉 ATCC16024
副溶血性弧菌 ATCC17802
单核增生李斯特菌 ATCC19115
缺陷短波单胞菌 ATCC19146
师岗链霉菌 ATCC19166
脱氮付球菌 ATCC19367
丝状链霉菌 ATCC19753
裂殖壶菌 ATCC20888
产朊假丝酵母 ATCC22023
乙酸钙不动杆菌 ATCC23055
珊瑚链霉菌 ATCC23901
菲律宾链霉菌 ATCC23905
里氏木霉 ATCC24449
变异链球菌 ATCC25175
沙链霉菌 ATCC25488
酪丁酸梭菌 ATCC25755
蛎灰链霉菌 ATCC27455
黄曲霉 ATCC28539
噬糖盐红菌 ATCC29252
隐甲藻 ATCC30772
干酪乳杆菌干酪亚种 ATCC334
休哈塔假丝酵母 ATCC34887
停乳链球菌 ATCC35666
昆明链霉菌 ATCC35682
扣囊内孢霉 ATCC36213
马克斯克鲁维酵母 ATCC36534
串珠镰孢 ATCC38946
米曲霉 ATCC42149
树状微杆菌 ATCC4358
潮湿纤维单胞菌 ATCC491
荧光假单胞菌 ATCC49642
水螺菌 ATCC49643
产气肠杆菌 ATCC49701
串珠镰孢 ATCC52539
金黄葡萄球菌 ATCC6538P
鼠李糖乳杆菌 ATCC7469
食油假单胞菌 ATCC8062
沙雷氏菌 ATCC8100
蛾微杆菌 ATCC8365
粪产碱菌 ATCC8750
枯草芽孢杆菌 ATCC9327
空肠弯曲杆菌ATCC 33291
产气肠杆菌ATCC13048
金黄葡萄球菌ATCC 27217
阴沟肠杆菌ATCC 13047
阪崎肠杆菌ATCC 29544
弗氏柠檬酸杆菌ATCC 43864
肺炎克雷伯氏菌ATCC 10031
小肠结肠炎耶尔森氏菌ATCC 23715
亚利桑那沙门氏菌CMCC(B) 47001
克柔假丝酵母菌ATCC 14243
光滑念珠菌ATCC 15126
创伤弧菌ATCC 27562
