α-MEM（含谷氨酰胺、含酚红）

【Product Description】                       
Minimum Essential Medium Eagle (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. It does not contain deoxyribonucleosides and ribonucleosides. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
  【Storage and Stability】
MEM-Alpha should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of  liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/ granulation/ particulates precipitates or sediments ;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.

【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.

【Quality Control】
Minimum Essential Medium-Alpha, with L-Glutamine ，without phenol red is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.

【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.