产品描述
GeneDirex提供的高纯度、冻干的外泌体exoUlt具有卓越的稳定性,适用于多种应用,包括:检测校准、外泌体定量的Spike in control、不同技术的蛋白质标记分析,如蛋白质印迹和流式细胞术实验、外泌体制RNA和DNA的提取和分析。每瓶含外泌体颗粒数量>4x1011。
制备方法
分离包括切向流过滤和尺寸排阻色谱。通过微流体电阻脉冲传感(MRPS)对外泌体(小型EV)的蛋白质含量和颗粒数量进行定量和验证。
应用
Western Blot, ELISA, Electron Microscopy, Flow Cytometry, Nucleic Acid Extraction
产品参数规格:2 mL / 4×10¹¹ exosomes/ml浓度:4×10¹¹ exosomes/ml储存::Store at 4~25°C.复溶说明:Add 2 mL deio
nized water and resuspend exosomes pipetting the solution up and down 10 times till freeze-dried powder dissolved, avoiding bubbles.内含物: Lyophilized from human umbilical cord 检测结果

↑ Western Blot: Exosome Standards (Human Umbilical Cord) [EXO001-0002] - Marker detection by Western Blotting. Reco
nstituted Exosomes can be directly detected by transmembrane proteins (CD9/CD63/CD81) and cytosolic proteins in EVs (Flotillin/ALIX) and Non-EV co-isolated structures (ApoE/ApoA1)

↑ Cryogenic electron microscopy: Exosome Standards (Human Umbilical Cord) [EXO001-0002]