3T3-L1小鼠胚胎成纤维细胞

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上海禾午生物科技有限公司
17317861055 021-57886085
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  • Brand  禾午生物
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  • Main Business:质粒,菌株,标准品,细胞,试剂
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Last Updated 2025-08-12 14:34

3T3-L1小鼠胚胎成纤维细胞

货号:C23032
名称:3T3-L1(小鼠胚胎成纤维细胞)
种属:小鼠
年龄(性别):胚胎
组织来源:胚胎
生长特性:贴壁
细胞形态:成纤维细胞样
3T3-L1细胞是通过克隆分离得到的3T3(swiss小白鼠)的连续亚株。当3T3-L1细胞从快速分裂到长满且接触抑制时,
3T3-L1细胞经过前脂肪向脂肪样逆转。培养液中,高血清含量可以促进3T3-L1细胞内脂肪的积累。
培养条件:气相:空气,95%;CO2,5%
温度:37℃10% CS /贴壁 
生长培养基:DMEM(PM150210) + 10% FBS(164210-500) + 1% P/S(PB180120)
推荐传代比例:1:3-1:4
推荐换液频率:2~3次/周
冻存液: 55%基础培养基+ 40%FBS+ 5%DMSO
受体表达情况:insulin, expressed
保藏机构:ATCC; CL-173 ATCC; CCL-92.1 BCRC; 60159
Company Name 上海禾午生物科技有限公司
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Business Scope 质粒,菌株,标准品,细胞,试剂
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