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重组小鼠NF-κB受体激活因子配体（RANKL）
PRP1114 | Mouse RANKL protein
产品概述
本品为无标签重组蛋白，氨基酸序列来源于小鼠RANKL（O35235）Pro143-Asp316片段，经大肠杆菌表达系统制备，纯度≥98%（SDS-PAGE验证）。适用于细胞培养领域的功能性ELISA检测，特异性结合人OPG的EC50值为55 ng/mL。
关键参数
- 活性验证：功能性ELISA检测显示与OPG(C-Fc)结合，EC50=55 ng/mL
- 分子量：19 kDa（还原条件SDS-PAGE）
- 内毒素：＜1.0 EU/μg（LAL法测定）
- 保存形态：冻干粉（PBS缓冲液，pH7.4）
技术规格
| 种属来源 | 小鼠 |
| 表达宿主 | 大肠杆菌 |
| 蛋白长度 | 174个氨基酸（N-Met） |
| 溶解建议 | 推荐使用配套复溶缓冲液（浓度≥100 µg/mL） |
保存与运输
- 冻干粉：＜-20℃长期保存
- 复溶后：4℃保存2-7天，-20℃长期保存（建议添加0.1%载体蛋白）
- 运输条件：常温运输（含冰袋保温）
产品规格
5 μg ¥588 | 20 μg ¥1498 | 100 μg ¥4698 | 1 mg 请询价
所有规格均现货供应，订单截止时间前确认可次日发货
注意事项
※ 本品仅供科研使用，严禁用于临床诊断或人体实验
※ 使用前请离心处理冻干粉，建议分装保存以避免反复冻融
相关资源
UniProt序列：O35235
基因ID：21943 | 别名：TNFSF11/TRANCE/OPGL
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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Recombinant Mouse Receptor Activator of NF-κB Ligand (RANKL)
PRP1114 | Mouse RANKL protein
Product Overview
This product is a tag-free recombinant protein derived from the Pro143-Asp316 fragment of mouse RANKL (O35235), expressed in E. coli expression system with ≥98% purity (verified by SDS-PAGE). Suitable for functional ELISA assays in cell culture applications, exhibiting specific binding to human OPG with an EC50 value of 55 ng/mL.
Key Parameters
- Activity Validation: Functional ELISA demonstrates binding to OPG(C-Fc) with EC50=55 ng/mL
- Molecular Weight: 19 kDa (reducing SDS-PAGE)
- Endotoxin: ＜1.0 EU/μg (LAL assay)
- Formulation: Lyophilized powder (PBS buffer, pH7.4)
Technical Specifications
| Species | Mouse |
| Expression Host | E. coli |
| Protein Length | 174 amino acids (N-Met) |
| Reconstitution | Recommended to use provided buffer (concentration ≥100 µg/mL) |
Storage & Shipping
- Lyophilized: Long-term storage at ＜-20℃
- Reconstituted: 4℃ for 2-7 days, long-term storage at -20℃ (0.1% carrier protein recommended)
- Shipping: Ambient temperature (with ice packs)
Product Specifications
5 μg ¥588 | 20 μg ¥1498 | 100 μg ¥4698 | 1 mg Contact for pricing
All specifications are in stock and available for next-day shipment if ordered before cutoff time
Notes
※ For research use only. Not for clinical diagnosis or human experimentation
※ Centrifuge lyophilized powder before use. Aliquot recommended to avoid repeated freeze-thaw cycles
Related Resources
UniProt Sequence: O35235
Gene ID: 21943 | Aliases: TNFSF11/TRANCE/OPGL
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Note: The above information may be subject to changes due to product optimization and upgrades. Please contact AcroBiosystems for detailed information.
