HiCrome™ 大肠菌琼脂

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上海嵘崴达实业有限公司
13788995069 021-64133189
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  • Main Business:进口原装生化试剂,抗体,耗材
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Last Updated 2025-08-12 14:37

HiCrome™ 大肠菌琼脂

大肠杆菌琼脂

Coliform ChromoSelect Agar

for microbiology, NutriSelect Plus

别名: HiCrome 大肠菌琼脂, 大肠杆菌琼脂, 大肠菌琼脂 HiCrome

General description

大肠菌群颜色选择琼脂是一种显色混合物,其含有两种生色底物,Salmon-GAL和X-葡萄糖醛酸苷。大肠杆菌可产生一种叫做β-D-半乳糖苷酶的酶,它可以切割Salmon-GAL,从而使大肠菌群呈橙红色至红色。由 大肠杆菌 产生的β-D-葡萄糖醛酸酶可切割X-葡萄糖醛酸苷。由于同时对Salmon-GAL和X-葡糖苷酸的切割,因而 大肠杆菌 会形成深蓝色至紫色的菌落。 色氨酸的添加可改善吲哚反应,从而提高了与两种色原体组合的检测可靠性。为了确认 大肠杆菌,可在深蓝色至紫色的菌落上加入一滴Kovac试剂(货号60983)。如果有樱桃红色的形成,则表明为阳性反应。

Application

大肠菌群颜色选择琼脂是一种选择性显色培养基,推荐用于同时检测 大肠杆菌 和水和食物样本中的总大肠菌群。

Preparation Note

Suspend 27 g in 1 litre distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. When a high number of gram-positive accompanying bacteria are expected, add 5 mg/l Novobiocin (Cat. No. 74675) before autoclaving of the medium.

Reconstitution

将27g悬浮于1L蒸馏水中。加热至沸腾以完全溶解培养基。通过在121℃下高压灭菌15分钟进行灭菌。当如果预期有大量革兰氏阳性伴随细菌时,在培养基高压灭菌前加入5mg/L新生霉素(目录号74675)。
 

       上海嵘崴达实业有限公司,专业经营生命科学领域的科研试剂、仪器和实验室消耗品,

致力于向国内生命科学研究人员提供合格产品和完善服务。

       主要经营的试剂品牌:
Roche试剂,部分常用现货,周三定货,2-3周到货。
GE试剂,耗材,部分常用现货,周二,周五定货,3周左右。
SIGMA试剂,周一,五定货,3周左右到货。
THERMO试剂,部分现货,周三定货,2-3周到货。
ABCAM试剂,国内现货三天左右,期货四周左右。
Peprotech试剂,部分常用现货,周三定货,2-3周到货。

罗氏原料,请来电来邮咨询。

OXOID,BD培养基础货期八周左右,部分现货。
MCE激动剂,ACROS、ALFA、阿拉丁等生化试剂现货定购。

Company Name 上海嵘崴达实业有限公司
Location
Business Scope 进口原装生化试剂,抗体,耗材
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Medicine Platform
 
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