| Background: MEF cells serve as feeder cells that support the growth of undifferentiated |
| mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). |
| MEF cells are isolated from mouse embryos and should be used at their early passages. |
| Before use, MEF cells are usually treated by γ-irradiation or mitomycin-C to stop their further |
| Product description: Neo MEF cells are derived from mice that are genetically engineered |
| with Neomycin-resistant genes. |
| Source: 13.5-day old DR4 mouse embryos |
| ESC-Sure™ DMEM (Applied StemCell: Cat # ASM-5001) |
| 10% ESC-Sure™ FBS (Applied StemCell: Cat# ASM-5007) |
| 1x NEAA (Invitrogen: Cat# 11140050) |
| 1x sodium pyruvate (Gibco Cat#11360-070) |
| 1x L-glutamine (Invitrogen: Cat# 25030164) |
0.1 mM beta-mercaptoethanol (Invitrogen: Cat# 21985023)
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