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0.25%胰酶溶液(含 EDTA,含酚红)

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  • 10.83/

上海雅酶生物医药科技有限公司
18701893452 021-34781650
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  • Brand  雅酶货号CB011
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Brand 雅酶货号CB011
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Last Updated 2025-08-12 14:38

0.25%胰酶溶液(含 EDTA,含酚红)

0.25%胰酶溶液(含 EDTA,含酚红)
Trypsin-EDTA (0.25%), phenol red
本产品干冰运输;-20℃避光保存,保质期12个月

 

货号规格

goodCB011   100mL

 

产品简介

good含酚红,含2.5g/L胰酶(1:250)溶于HBSS溶液,0.02%EDTA溶HBSS溶液,不含钙、镁。

 

产品概述

good一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。由于EDTA能够螯合Ca2+和Mg2+,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。
good胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在PH 8.0和37℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。

 

注意事项

good1.由于不同的组织或者细胞对胰酶的作用反应不一样,因此操作人员应根据实际情况,确定最佳消化时间;消化细胞时间不宜过长,否则会影响细胞贴壁和生长状况;
good2.谨慎操作,避免污染;
good3.2℃~8℃中解冻,摇匀后使用,切忌反复冻融,用量较少时建议分装冻存;
good4.由于D-Hank's平衡盐溶液NaHCO3含量较低(0.35g/L),因此该型胰蛋白酶溶液不能用于5% CO2的环境,若放入CO2培养相,溶液将迅速变酸,使用时应注意;
good5.本产品仅供科研使用,不可用于医药、临床等领域。

 

Company Name 上海雅酶生物医药科技有限公司
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