JJN-3

• Cat.No.
• CSC-C0549

• Description
• Established from the bone marrow of a 57-year-old woman with plasma cell leukemia (IgA1kappa) at diagnosis in 1987; JJN-3 is a subclone of the parental cell line JJN-1; plasma cell leukemia is related to multiple myeloma

• Recommended Medium
• 40% DMEM + 40% IMDM + 20% h.i. FBS

• Organism
• Human

• Morphology
• single round to oval cells showing a morphological range from mononuclear to occasionally multinucleated cells growing in suspension

• Culture Properties
• suspension

• Karyotype
• Human flat-moded hypotriploid karyotype with 9% polyploidy - 58-67<3n>XX, +1, -2, +5, +8, +8, -9, -11, -12, -13, -15, -17, +20, add(1)(p22), der(1)t(1;?3)(q41;p26)x2, add(3)(p26), add(5)(p15)x1-2, i(5p), del(6)(q13),del(7)(q32), der(7)t(7;11)(q36;q13), ad

• Quality Control
• Mycoplasma: negative in microbiological culture, PCR assays
  Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, cykappa +, sm/cylambda -
  Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II

• Storage and Shipping
• Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

• Tissue
• plasma cell leukemia