A validation product for biological mycoplasma as well as for mycoplasma co
ntamination in the production cell strain
- Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- The primer binds to the highly conserved 16s rRNA coding region
- DNA of eukaryotes and other bacterial species are not detected
- PCR performance monitoring is possible with the application of internal amplification control (IAC)
- Optimized PCR Premix provided for easy use
- Using the TaqMan probe method, real-time PCR (qPCR) technique is applied
(FAM labeled probe: Mycoplasma, HEX labeld probe: IAC) - Main strand DNA preparation using cell culture solution or commercial DNA extraction kit
- Sensitivity: 1 ~ 10 fg/reaction
- Result confirmation within 1–2 hr
- Passed the Korea Ministry of Food and Drug Safety (MFDS), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP) standards
- UDG system application: Prevention of carry-over contamination
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