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细胞活性测定试剂盒

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上海逍鹏生物科技有限公司
18918495545 4008203979
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Last Updated 2025-08-12 14:50

细胞活性测定试剂盒

品名:细胞活性测定试剂盒
型号:20-300-1000
描述:细胞活性测定试剂盒

产品名称 货号 规格 储存条件
Cell Proliferation Kit(XTT based) 细胞活性测定试剂盒(二甲氧唑黄) 20-300-1000 1000 assays -20ºC

细胞活性测定试剂盒广泛应用于细胞生物学的各类实验中,例如研究生长因子、细胞活素类和培养基组分,细胞毒性因子和淋巴细胞的活性。 MTT和XTT都是检测细胞存活和生长的方法,MTT的产物为水不溶性的蓝紫色结晶甲瓒(Formazan),并沉积在细胞中,需要加有机溶剂溶解,由于在去上清的操作时会有可能带走小部分的甲瓒,故重复性略差。XTT作为线粒体脱氢酶的作用底物,被活细胞还原成水溶性的橙黄色甲臢产物,当XTT与电子偶合剂(例如PMS)联合应用时,其所产生的水溶性的甲臢产物的吸光度与活细胞的数量成正比。

XTT优点: 1. 使用方便,省去了洗涤细胞 2. 检测快速
3. 灵敏度高,甚至可以测定较低细胞密度 4. 重复性优于MTT 细胞活性测定试剂盒适用于单核细胞(Monocytes),人肝癌细胞Hep G2胚胎纤细胞 Ehd-1, 小鼠纤维细胞,恶性黑色素瘤A375,胰腺癌BXPC-3等30余种细胞类型。
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