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Product description:
Recombinant human EGF-Fc protein was expressed from the mature soluble form of human EGF (Asn971-Arg1023, Accession # P01133) fused with GST tag at its N-terminus. The predicted molecular weight is about 33.5kDa. The recombinant protein was purified by purified by conventional chromatography techniques. Purity is greater than 90% by SDS-PAGE and Coomassie blue staining (Figure 1).
Formulation:
Lyophilized from a 0.22μm filtered solution at a concentration of 1mg/mlin PBS.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 1.0 mg/ml.
Shipping&Stablity:
The Product is shipped at ambient temperature. Upon reconstitution, the preparation is stable for up to 1 month at 2-8°C. For long term storage, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles
Figure1. The purity of recombinant protein GST-EGF (AZ-007). 12 % SDS-PAGE, 2μg protein under reducing condititon .
For ordering and further information on this or other AbZyme products, contact customer Services
| Recombinantprotein | Species | Catalog# | Amount |
| GST-EGF | human | AZ-032-50 | 50μg |
| GST-EGF | human | AZ-032-100 | 100μg |
| GST-EGF | human | AZ-032-1mg | 1mg |
Backgroud:
EGF (epidermal growth factor) is a mitogenic polypeptide with 53 amino acid residuesand three intramolecular disulfide bonds. EGF plays an important role in the regulation of cell growth, proliferation, and differentiation. EGF can promote wound healing and organogenesis. EGF is commonly used as a supplement in serum-free or reduced serum media for culture of mammalian cells. EGF is applicable to cosmetics such as whitening and crinkle prevention.
