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Product description:
Recombinant human EGF-Fc protein was expressed from the mature soluble form of human EGF (Asn971-Arg1023, Accession # P01133) fused with Fc region of human IgG1 at its C-terminus. The chimera was produced in a mammalian cell line. A recombinant EGF-Fc is sulfide-linked homodimer. The predicted molecular weight is about 33kDa under reducing condition. This recombinant protein was purified by affinity chromatography. Purity is greater than 85% by SDS-PAGE (Figure 1).
GenBank Accession number:P01133
Formulation:
Lyophilized from a 0.22μm filtered solution at a concentration of 1mg/mlin PBS.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 1.0 mg/ml.
Shipping&Stablity:
The Product is shipped at ambient temperature. Upon reconstitution, the preparation is stable for up to 1 month at 2-8°C. For long term storage, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Figure 1.The purity of recombinant EGF-Fc (AZ-m008).12% SDS-PAGE analysis of 5µg of EGF-Fc under reducing condition.
For ordering and further information on this or other AbZyme products, contact customer Services.
| Product | Species | Catalog# | Amount |
| EGF-Fc | human | AZ-m008-200 | 200μg |
| EGF-Fc | human | AZ-m008-500 | 500μg |
| EGF-Fc | human | AZ-m008-1mg | 1mg |
Background:
EGF (epidermal growth factor) is a mitogenic polypeptide with 53 amino acid residuesand three intramolecular disulfide bonds. EGF plays an important role in the regulation of cell growth, proliferation, and differentiation. EGF can promote wound healing and organogenesis. EGF is commonly used as a supplement in serum-free or reduced serum media for culture of mammalian cells. EGF is applicable to cosmetics such as whitening and crinkle prevention.
