GRF102 磷酸酶抑制剂混合液(100×）

磷酸酶抑制剂混合液(100×)
Phosphatase Inhibitor Cocktail(100×)
本产品冰袋运输；保存于-20℃，保质期12个月。

货号规格
goodGRF102(100×，水溶液)  1mL

产品特点
gd高效全面——多达8种的高效抑制成分，提供比同类产品(一般含3~4种抑制剂)更为全面的磷酸酶抑制能力。

产品简介
good本品是一种广谱磷酸酶抑制剂，高效抑制Serine/Threonine Phosphatase，Tyrosine Phosphatase，Acid Phosphatase，Alkaline phosphatase等细胞内所含的磷酸酶。

成分说明
good含 Sodium fluoride ， Sodium orthovanadate ， Sodium molybdate ， Sodium tartrate ，Imidazole ， Sodium pyrophosphate decahydrate ， β-Glycerophosphate ， Cantharidin 等8种高效磷酸酶抑制剂。

使用说明

good1. 本品用于各种蛋白质含量或其活性分析实验(Western Blot，Co-IP，pull-down，IF，IHC等)，抑制磷酸酶的活性； good2. 将本品以1:100(体积比)的稀释比加入到目标溶液中(如细胞裂解液，蛋白提取液等)。

注意事项

good1. 当与蛋白酶抑制剂联用时，请不要使用含有EDTA的蛋白酶抑制剂，因为EDTA会严重影响 磷酸酶抑制剂混合液 中某些成分的抑制效果。事实上 磷酸酶抑制剂混合液 中已含有多种螯合剂成分； good2. 本品为高浓度溶液，长期存放可能会出现微量晶体状沉淀，此为正常现象，不影响实验结果； good3. 不同来源的样品中磷酸酶含量各不相同，1:100为推荐的常规剂量。实际使用中，如发现磷酸酶含量较高，可适当提高 磷酸酶抑制剂混合液 的使用浓度； good4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。所有抑制剂对人体均有毒，请勿直接与皮肤接触。如不小心接触皮肤，请立即用大量清水冲洗。如不慎吞食，应立即就医处理； good5. 本产品仅限科研使用。相关论文 good1. Huang, Y., Chen, S., Wu, S., Dong, G., & Sheng, C. (2020). Evodiamine-inspired dual inhibitors of histone deacetylase 1 (HDAC1) and topoisomerase 2 (TOP2) with potent antitumor activity. Acta Pharmaceutica Sinica B, 10(7), 1294-1308. （IF 11.413）
good2. Wang F, Li Z, Zhou J, et al. SIRT1 regulates the phosphorylation and degradation of P27 by deacetylating CDK2 to promote T-cell acute lymphoblastic leukemia progression[J]. Journal of Experimental & Clinical Cancer Research, 2021, 40(1): 1-16.（IF 11.161）
good3. Jiang Z, Wang Y, Li Z, et al. The m6A mRNA demethylase FTO in granulosa cells retards FOS-dependent ovarian aging[J]. Cell death & disease, 2021, 12(8): 1-10.（IF 8.469）
good4. Hua T, Wang H, Fan X, et al. BRD4 Inhibition Attenuates Inflammatory Pain by Ameliorating NLRP3 Inflammasome-Induced Pyroptosis[J]. Frontiers in immunology, 2022, 13: 837977-837977.（IF 7.561）
good5. Zhu Y, Tang X, Xu Y, et al. RNASE2 Mediates Age-Associated B Cell Expansion Through Monocyte Derived IL-10 in Patients With Systemic Lupus Erythematosus[J]. Frontiers in immunology, 2022, 13: 752189-752189.（IF 7.561）
good6. Cheng, J., Li, Y., Wang, X., Dong, G., & Sheng, C. (2020). Discovery of Novel PDEδ Degraders for the Treatment of KRAS Mutant Colorectal Cancer. Journal of Medicinal Chemistry, 63(14), 7892-7905. （IF 7.446）
good7. Pan, C., Chen, L., Wu, R., Shan, H., Zhou, Z., Lin, Y., ... & Wu, C. (2018). Lithium-containing biomaterials inhibit osteoclastogenesis of macrophages in vitro and osteolysis in vivo. Journal of Materials Chemistry B, 6(48), 8115-8126. （IF 6.331）
good8. Xu Z, Shi L, Li D, et al. Real ambient particulate matter-induced lipid metabolism disorder: Roles of peroxisome proliferators-activated receptor alpha[J]. Ecotoxicology and Environmental Safety, 2022, 231: 113173.（IF 6.291）
good9. Pan, C., Shan, H., Wu, T., Liu, W., Lin, Y., Xia, W., ... & Yu, X. (2019). 20 (S)-protopanaxadiol inhibits titanium particle-induced inflammatory osteolysis and RANKL-mediated osteoclastogenesis via MAPK and NF-κB signaling pathways. Frontiers in pharmacology, 9, 1538. （IF 5.810）
good10. GAO, Mengyu, et al. The Role of Nrf2 in the PM-Induced Vascular Injury Under Real Ambient Particulate Matter Exposure in C57/B6 Mice. Frontiers in pharmacology, 2021, 12: 206.（IF 5.810）
good11. Cui, L., Shi, L., Li, D., Li, X., Su, X., Chen, L., ... & Chen, C. (2020). Real-ambient particulate matter exposure-induced cardiotoxicity in C57/B6 mice. Frontiers in Pharmacology, 11, 199. （IF 5.810）
good12. Zhu Y, Tao Z, Chen Y, et al. Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT[J]. Breast Cancer Research and Treatment, 2022: 1-17.（IF 4.872）
good13. Cai, Y., Li, X., Pan, Z., Zhu, Y., Tuo, J., Meng, Q., ... & Pan, Y. (2020). Anthocyanin ameliorates hypoxia and ischemia induced inflammation and apoptosis by increasing autophagic flux in SH-SY5Y cells. European Journal of Pharmacology, 883, 173360. （IF 4.432）
good14. He Z, Liu X X, Liu X, et al. The role of MEG3 in the proliferation of palatal mesenchymal cells is related to the TGFβ/Smad pathway in TCDD inducing cleft palate[J]. Toxicology and Applied Pharmacology, 2021, 419: 115517.(IF 4.219)
good15. Lv, N., Yuan, J., Ji, A., Shi, L., Gao, M., Cui, L., & Jiang, Q. (2019). Perfluorooctanoic acid-induced toxicities in chicken embryo primary cardiomyocytes: Roles of PPAR alpha and Wnt5a/Frizzled2. Toxicology and applied pharmacology, 381, 114716. （IF 4.219）
good16. Fan, Z., Luo, H., Zhou, J., Wang, F., Zhang, W., Wang, J., ... & Liang, A. (2020). Checkpoint kinase‑1 inhibition and etoposide exhibit a strong synergistic anticancer effect on chronic myeloid leukemia cell line K562 by impairing homologous recombination DNA damage repair. Oncology reports, 44(5), 2152-2164.（IF 3.906）
good17. Huang, D., Xiong, M., Xu, X., Wu, X., Xu, J., Cai, X., ... & Zhou, H. (2020). Bile acids elevated by high-fat feeding induce endoplasmic reticulum stress in intestinal stem cells and contribute to mucosal barrier damage. Biochemical and Biophysical Research Communications, 529(2), 289-295. （IF 3.575）
good18. LIU, Xiaozhuan, et al. The role of lncRNA Meg3 in the proliferation of all-trans retinoic acid-treated mouse embryonic palate mesenchymal cells involves the Smad pathway. Reproductive Toxicology, 2021.（IF 3.143）
good19. Wang Y, Chen X, Yao N, et al. MiR-448 suppresses cell proliferation and glycolysis of hepatocellular carcinoma through inhibiting IGF-1R expression[J]. Journal of Gastrointestinal Oncology, 2022, 13(1): 355.（IF 2.892 ）
good20. WU, Hailun, et al. α-Tomatine, a novel early-stage autophagy inhibitor, inhibits autophagy to enhance apoptosis via Beclin-1 in Skov3 cells. Fitoterapia, 2021, 152: 104911.（IF 2.882）