Recombinant Human TPBG/5T4 (C-

Recombinant Human TPBG/5T4 (C-mFc)(C01X)
 

 

产品说明(Description)

Recombinant Human Trophoblast glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a mFc tag at the C-terminus. 

Accession #: Q13641

Known as: TPBG; 5T4 ; 5T4AG; M6P1; WAIF1

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
 

质量控制(Quality Control) 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
 

复溶（Reconstitution）

Always centrifuge tubes before opening.  Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.   

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY