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xGen DNA Lib Prep EZ 文库制备试剂盒

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上海仁科生物科技有限公司
021-34250079
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  • Brand  IDT
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Last Updated 2025-08-12 15:11

xGen DNA Lib Prep EZ 文库制备试剂盒

一盒建库、流程灵活、应用无限

xGen DNA Lib Prep EZ 文库制备试剂盒可通过双链 DNA 简便地制备高品质的下一代测序(NGS) 文库。该试剂盒适于在llumina 平台上,通过需要或不需要 PCR 扩增及靶向测序的方式,采用酶切片段法建立文库。它将片段切割、末端修复、dA尾添加、接头连接以及微珠纯化步骤全部纳入一次单管反应,从而将样本操作和总体文库制备时间缩短为大约 2 小时图 1)。该试剂盒还兼容多种 DNA 投入量 (1-250 ng),偏倚低,可达到均一的序列覆盖 (图 2)。
这种模块化试剂盒可让您根据自己的应用需求定制文库。例如:
片段大小 - 通过孵育时间来控制切割片段的大小
接头和标记法 - 使用标准 TA 连接建库法,连接 P5 和 P7 接头(本试剂盒中不含)。可使用全长接头或短接头进行定制,并采用您选择的任何样本标记法。
建库流程灵活 - PCR 为可选步骤,具体取决于您要求的接头或样本投入量。

 

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