Language
产品介绍 Background:
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the FGF superfamily of mitogenic proteins which show 35-60% amino acid conservation[1]. FGF acidic and basic are unique from other members of the family in that they lack classical secretory signal peptides. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury[2]. FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may also participate in the development of several pathological conditions resulting from excessive cell proliferation and/or angiogenesis[3]. BFGF is a critical component of human embryonic stem cell culture medium[4].
技术参数 Source:
E.coli
Protein Construction:
A DNA sequence encoding the amino acids (Pro143-Ser288) of human FGF-2 (Accession Number:
P48798) was expressed.
Synonyms:
Basic fibroblast growth factor; bFGF; FGF basic; FGF-2.
SPECIFICATIONS
Purity:
≥ 97%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Biological Activity:
Measured in CellTiter-Glo 3D Cell Viability Assay using human bronchoalveolar organoids. The ED50 for this effect is 10-50 ng/ml.
Endotoxin Level:
<0.10 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:
16.5 kDa
SDS-PAGE:
18 kDa, reducing conditions
DATA
FORMULATION AND STORAGE
Formulation:
The product is Lyophilized from a 0.22 μm filtered solution in PBS.
Shipping:
The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitution:
Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:
24 months, -20 to -70 °C, under powder state;
12 months, -20 to -70 °C, under sterile conditions after reconstitution;
2 month, 2 to 8 °C under sterile conditions after reconstitution;
avoid repeated freeze-thaw cycles.
