Language
说明：脂肪组织特别是白色脂肪组织（WAT）已经被证实除了具有能量储存功能外，还与内分泌和器官炎症有关。从脂肪组织中提取和分析蛋白质对于了解许多生理/病理状态越来越重要。但是由于白色脂肪组织（WAT）和棕色脂肪组织（BAT）中高脂肪和低蛋白含量，所以在技术上极具挑战性。众所周知目前生物样品中水油乳化物最难分离，我公司研发的具有独特表面性质的带孔径的离心管柱和优化的无表面活性剂的缓冲液系统，可以快速有效的从脂肪组织匀浆中将水油乳化物分离。提取缓冲液比脂肪组织中的油冰点低，脂肪组织匀浆通过离心管柱能将水相和油相迅速分开，组织中的总蛋白无丢失。蛋白质得率可达2-3 mg/ml，远远高于其他方法。
特点：
- 速度快，操作简单，可靠
- 无蛋白丢失，高得率
- 适用于不同类型的脂肪组织和细胞
- 动物脂肪组织及细胞中提取蛋白的商业试剂和盒
- 储存条件：Buffer A储存于4℃，其他部分15-30℃
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