EMSA试剂盒

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北京擎科生物科技股份有限公司
18622657960 00000000
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  • Brand  达领
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Last Updated 2025-08-12 15:19

EMSA试剂盒

产品简介:凝胶阻滞或电泳迁移率实验(Electrophoretic mobility shift assay,EMSA)是一种体外研究DNA结合蛋白质(包括转录因子、共调节因子)和DNA相互作用的技术。EMSA基于DNA-蛋白质复合体在聚丙x烯x酰胺凝胶电泳(PAGE)中迁移率的不同,当DNA结合蛋白质与DNA结合后,其在PAGE中的迁移率将小于未结合蛋白DNA,从而根据显影结果判定两者间是否结合。
产品应用:
♦ 鉴定蛋白-DNA结合位点,确定某一转录因子能否结合具体DNA序列;
♦ 分析结合特异性:通过变异DNA序列或竞争实验,评定结合区域的特异残基;
♦ 转录因子活性对比研究。
产品特点:
♦ 转录因子(包括转录因子、共调节因子)靶基因研究; 
♦ 转录因子在靶基因启动子区域结合位点验证研究;
♦ 组间转录因子活性对比研究。

Company Name 北京擎科生物科技股份有限公司
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