抗体标记试剂盒，Lightning-Link™ “一步法”

英国Innova Biosciences公司是一家专注于生物偶联技术（bioconjugation）的企业。生物偶联即将两种分子结合在一起而形成一个杂合的偶联物 （hybrid conjugate），这些偶联结合物（如标记抗体）在世界各大医院和科研机构被广泛使用。InnovaBiosciences公司开发生产的“一步法” 抗体标记试剂盒-Lightning-Link™系列产品极大程度的简化了结合标记流程，是目前世界上操作最简便的抗体标记试剂盒。
Lightning-Link™ “一步法”抗体标记试剂盒

在 进行多因子检测时，我们往往因不能找到多种合适的标记一抗，而不得不选用标记二抗，因此很难避免可能发生的交叉反应及繁琐的洗涤步骤。 Lightening-LinK™抗体标记试剂盒系列，提供多种荧光蛋白、酶及荧光素标记等产品，试验操作人员可直接应用于标记一抗，从而避免了因使用二 抗带来的交叉反应及非特异性结合等问题。该系列试剂盒具有以下显著特点：

特点	优势
操作简单：一步法标记 快速: 手工操作时间仅30秒	目前世界上操作最简便的抗体标记试剂盒
无需脱盐、透析等步骤	抗体回收率达100%
可提供数十种标记物	可用于WB,IHC，ELISA,免疫荧光、流式细胞术以及荧光共振能量转移检测等
经济实惠，规格多样	可标记ug-mg 级抗体量

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Innova Bioscience公司Lighting-Link™系列与其他品牌的抗体标记试剂盒比较
厂 家	Supplier I1	Supplier I2	Supplier I3	Supplier S1	Supplier S2	Supplier D	Lighting-Link
手工操作时间	2 分钟	20分钟	30分钟	30分钟	30分钟	10分钟	30秒钟
是否过柱	不需要	需要	需要	需要	需要	需要	不需要
试剂盒组分	6组分	7组分	5组分	9组分	12组分	6组分	3组分
操作步骤	5步	11步	16步	27步	31步	8步	3步
抗体标记时间	10分钟	2.5小时	2小时	1.5小时	4小时	1小时	2小时
可标记抗体量	1-20ug	10-20ug	20-100ug	25ug-5mg	100ug	50-200ug	100ug-5mg
连接化学健	非共价结合*	共价结合	共价结合	共价结合	共价结合	共价结合	共价结合
抗体亚型	仅IgG	仅IgG	所有	仅IgG	仅IgG	仅IgG	所有

*非共价连接的抗体标记物只能稳定30分钟。
试剂盒组成：
1）1或3(以规格而定)玻管Lightening-Link™ Mix 混合物。
2）1管LL-X（X为标记物，如PE或HRP等）修饰试剂。
3）1管LL-X萃灭试剂。
操作步骤：
1）在每10ul抗体样品中加入1ul的LL-Modifier修饰试剂，轻轻混匀。
2）打开Ligtening-Link混合物管盖，吸取步骤1已混好的抗体样品至管内冻干混合物中，用吸液器轻轻吸打1-2次,使粉末完全溶解。
3）盖上玻璃瓶盖，室温(20-25°C)避光孵育3小时,或过夜。*
4）孵育3小时后，按每10ul抗体的量加入1ul LL-淬灭试剂，静置30分钟后即可使用。
*不同的标记物，操作步骤略有不同。（操作步骤供参考，以英文原版说明书为准。）
标记结合物的保存：
对于任何新结合物，推荐保持于4°C条件下。如需长期保存，可添加保护剂。
缓冲液及添加剂兼容性：
A. 与无胺基缓冲液兼容，包括MES,MOPS,HEPES（浓度10-50mM, pH范围6.5-8.5）,LL-Modifier修饰剂提供结合反应的最佳条件。
B. 常见的盐（如氯化钠）、螯合剂（EDTA）、糖对偶联反应无影响。
C. 叠氮钠（0.02%-0.1%）对结合反应有轻微影响，叠氮钠不可用于HRP标记，其为后者的抑制剂。
D. 甘油(up to 50%)对结合效率无影响。
E. 若无胺基的缓冲液浓度相对较高，pH值在6.6-8.5范围外，则需要加入更多的LL-midifier修饰剂（/10ul抗体），以此来调整pH值。最佳的pH范围为7.3-7.6左右。
G. 浓度达到或稍低于20mM的Tris对结合效率有轻微的影响。

产品编号	产品名称	规格
HRP辣根过氧化物酶抗体标记试剂盒
701-0010	Lightning-Link HRP conjugation kit	1 x 100μg HRP
701-0000	Lightning-Link HRP conjugation kit	3 x 100μg HRP
701-0002	Lightning-Link HRP conjugation kit	1mg HRP
701-0003	Lightning-Link HRP conjugation kit	5 x 1mg HRP
701-0004	Lightning-Link HRP conjugation kit	5mg HRP
AP碱性磷酸酶抗体标记试剂盒
702-0005	Lightning-Link AP conjugation kit	1 x 100μg AP
702-0010	Lightning-Link AP conjugation kit	3 x 100μg AP
702-0015	Lightning-Link AP conjugation kit	1mg AP
GOx葡糖氧化酶标记试剂盒
706-0010	Lightning-Link GOx conjugation kit	3 x 100μg GOx
706-0015	Lightning-Link GOx conjugation kit	1 x 1mg GOx
R-PE（PE）标记试剂盒
703-0005	Lightning-Link R-Phycoerythrin (R-PE)	1 x 100μg PE
703-0010	Lightning-Link R-Phycoerythrin (R-PE)	3 x 100μg PE
703-0015	Lightning-Link R-Phycoerythrin (R-PE)	1mg PE
B-PE标记试剂盒
716-0005	Lightning-Link B-Phycoerythrin (B-PE)	1 x 100μg BPE
716-0010	Lightning-Link B-Phycoerythrin (B-PE)	3 x 100μg BPE
716-0015	Lightning-Link B-Phycoerythrin (B-PE)	1mg BPE
C-PE标记试剂盒
720-0005	Lightning-Link C-Phycoerythrin (C-PE)	1x100ug
720-0010	Lightning-Link C-Phycoerythrin (C-PE)	3x100ug
720-0015	Lightning-Link C-Phycoerythrin (C-PE)	1x1mg
APC标记试剂盒
705-0005	Lightning-Link Allophycocyanin (APC)	1 x 100μg APC
705-0010	Lightning-Link Allophycocyanin (APC)	3 x 100μg APC
705-0015	Lightning-Link Allophycocyanin (APC)	1mg APC
C-PC标记试剂盒
709-0005	Lightning-Link C-Phycocyanin (C-PC)	1 x 100ug CPC
709–0010	Lightning-Link C-Phycocyanin (C-PC)	3 x 100ug CPC
709-0015	Lightning-Link C-Phycocyanin (C-PC)	1 x 1mg CPC
R-PC标记试剂盒
721-0005	Lightning-Link R-Phycocyanin (R-PC)	1x100ug
721-0010	Lightning-Link R-Phycocyanin (R-PC)	3x100ug
721-0015	Lightning-Link R-Phycocyanin (R-PC)	1x1mg
PerCP标记试剂盒
718-0005	Lightning-Link PerCP	1x100ug
718-0010	Lightning-Link PerCP	3x100ug
718-0015	Lightning-Link PerCP	1x1mg
荧光素标记试剂盒
707-0010	Lightning-Link Fluorescein	3 reactions (each up to 200ug)
707-0015	Lightning-Link Fluorescein	1 reaction (up to 2mg)
罗丹明标记试剂盒
710-0010	Lightning-Link Rhodamine	3 reactions (each up to 200ug)
710-0015	Lightning-Link Rhodamine	1 reaction (up to 2mg)
AMCA标记试剂盒
712-0010	Lightning-Link AMCA	3 reactions (each up to 200ug)
712-0015	Lightning-Link AMCA	1 reaction (up to 2mg)
Texas Red标记试剂盒
714-0010	Lightning-Link Texas Red	3 reactions (each up to 200ug)
714-0015	Lightning-LinkTexas Red	1 reaction (up to 2mg)
Cy系列染标记试剂盒
780-0010	Lightning-Link Cy3	3 reactions (each up to 200ug)
780-0015	Lightning-Link Cy3	1 reaction (up to 2mg)
781-0010	Lightning-Link Cy5	3 reactions (each up to 200ug)
781-0015	Lightning-Link Cy5	1 reaction (up to 2mg)
782-0010	Lightning-Link Cy5.5	3 reactions (each up to 200ug)
782-0015	Lightning-Link Cy5.5	1 reaction (up to 2mg)
783-0010	Lightning-Link Cy7	3 reactions (each up to 200ug)
783-0015	Lightning-Link Cy7	1 reaction (up to 2mg)
784-0010	Lightning-Link Cy3.5	3 reactions (each up to 200ug)
784-0015	Lightning-Link Cy3.5	1 reaction (up to 2mg)
Atto荧光染料标记试剂盒
730-0010	Lightning-Link atto390	3 reactions (each up to 200ug)
730-0015	Lightning-Link atto390	1 reaction (up to 2mg)
731-0010	Lightning-Link atto425	3 reactions (each up to 200ug)
731-0015	Lightning-Link atto425	1 reaction (up to 2mg)
732-0010	Lightning-Link atto465	3 reactions (each up to 200ug)
732-0015	Lightning-Link atto465	1 reaction (up to 2mg)
733-0010	Lightning-Link atto488	3 reactions (each up to 200ug)
733-0015	Lightning-Link atto488	1 reaction (up to 2mg)
736-0010	Lightning-Link atto532	3 reactions (each up to 200ug)
736-0015	Lightning-Link atto532	1 reaction (up to 2mg)
738-0010	Lightning-Link atto565	3 reactions (each up to 200ug)
738-0015	Lightning-Link atto565	1 reaction (up to 2mg)
739-0010	Lightning-Link atto590	3 reactions (each up to 200ug)
739-0015	Lightning-Link atto590	1 reaction (up to 2mg)
740-0010	Lightning-Link atto594	3 reactions (each up to 200ug)
740-0015	Lightning-Link atto594	1 reaction (up to 2mg)
741-0010	Lightning-Link atto610	3 reactions (each up to 200ug)
741-0015	Lightning-Link atto610	1 reaction (up to 2mg
742-0010	Lightning-Link atto611	3 reactions (each up to 200ug)
742-0015	Lightning-Link atto611	1 reaction (up to 2mg)
743-0010	Lightning-Link atto620	3 reactions (each up to 200ug)
743-0015	Lightning-Link atto620	1 reaction (up to 2mg)
744-0010	Lightning-Link atto633	3 reactions (each up to 200ug)
744-0015	Lightning-Link atto633	1 reaction (up to 2mg)
746-0010	Lightning-Link atto637	3 reactions (each up to 200ug)
746-0015	Lightning-Link atto637	1 reaction (up to 2mg)
749-0010	Lightning-Link atto655	3 reactions (each up to 200ug)
749-0015	Lightning-Link atto655	1 reaction (up to 2mg)

750-0010			Lightning-Link atto680	3 reactions (each up to 200ug)
750-0015			Lightning-Link atto680	1 reaction (up to 2mg)
751-0010			Lightning-Link atto700	3 reactions (each up to 200ug)
751-0015			Lightning-Link atto700	1 reaction (up to 2mg)
FluoProbes Dyes荧光探针染料标记试剂盒
788-0010	Lightning-Link FluoProbes 547H			3 reactions (each up to 200ug)
788-0010	Lightning-Link FluoProbes 547H			1 reaction (up to 2mg)
791-0010	Lightning-Link FluoProbes 647H			3 reactions (each up to 200ug)
791-0010	Lightning-Link FluoProbes 647H			1 reaction (up to 2mg)
792-0010	Lightning-Link FluoProbes 682H			3 reactions (each up to 200ug)
792-0010	Lightning-Link FluoProbes 682H			1 reaction (up to 2mg)
793-0010	Lightning-Link FluoProbes 752H			3 reactions (each up to 200ug)
793-0010	Lightning-Link FluoProbes7527H			1 reaction (up to 2mg)
PE/Cy系列串联染料
760-0005	Lightning-Link PE/Cy5			1x100ug
760-0010	Lightning-Link PE/Cy5			3x100ug
760-0015	Lightning-Link PE/Cy5			1x1mg
761-0005	Lightning-Link PE/Cy5.5			1x100ug
761-0010	Lightning-Link PE/Cy5.5			3x100ug
761-0015	Lightning-Link PE/Cy5.5			1x1mg
762-0005	Lightning-Link PE/Cy7			1x100ug
762-0010	Lightning-Link PE/Cy7			3x100ug
762-0015	Lightning-Link PE/Cy7			1x1mg
PerCP/Cy5.5串联染料
763-0005	Lightning-Link PerCP/Cy5.5			1x100ug
763-0010	Lightning-Link PerCP/Cy5.5			3x100ug
763-0015	Lightning-Link PerCP/Cy5.5			1x1mg
APC/Cy系列串联染料
764-0005	Lightning-Link APC/Cy5.5			1x100ug
764-0010	Lightning-Link APC/Cy5.5			3x100ug
764-0015	Lightning-Link APC/Cy5.5			1x1mg
765-0005	Lightning-Link APC/Cy7			1x100ug
765-0010	Lightning-Link APC/Cy7			3x100ug
765-0015	Lightning-Link APC/Cy7			1x1mg
生物素化结合试剂盒
704–0010	Lightning-Link Biotin conjugation kit type A			3 reactions (up to 200μg Ab)
704–0015	Lightning-Link Biotin conjugation kit type A			1 reaction (up to 2mg Ab)
715-0010	Lightning-Link Biotin conjugation kit type B			3 reactions (up to 200μg Ab)
715-0015	Lightning-Link Biotin conjugation kit type B			1 reaction (up to 2mg Ab)
链亲和素标记试剂盒
708-0010		Lightning-Link Streptavidin conjugation kit		3 x 100ug antibody
708-0015		Lightning-Link Streptavidin conjugation kit		1 x 1mg antibody
亲和素标记试剂盒
717-0010			Lightning-Link Avidin conjugation kit	3 x 100ug Avidin
717-0015			Lightning-Link Avidin conjugation kit	1 x 1mg Avidin
Trap fluorescein标记试剂盒
711-0001			Fluoro-Trap fluorescein conjugation kit	3 x 100μg antibody
711-0010			Fluoro-Trap fluorescein conjugation kit	5 x 1mg antibody
酶结合物稳定剂
901-0005			LifeXtend HRP conjugate stabilizer	50ml
902-0005			LifeXtend Alk phos conjugate stabilizer	50ml
马来酰亚胺活化的标记物（增强标记结合反应）
401-0005			Maleimide Activated HRP	5mgs
402-0005			Maleimide Activated Alkaline Phosphatase	5mgs
403-0005			Maleimide Activated R-Phycoerythrin	5mgs
404-0005			Maleimide Activated Allophycocyanin-XL	5mgs
405-0005			Maleimide Activated Streptavidin	5mgs
406-0005			Maleimide Activated Glucose Oxidase	5mgs
407-0005			Maleimide Activated Ovalbumin	5mgs
408-0005			Maleimide Activated BSA	5mgs
Colorlock磷检测试剂盒
302-0125			PiColorlock ALS	625/1560 assays
302-0500			PiColorlock ALS	2500/6250 assays
303-0030			PiColorlock Gold	625/1560 assays
303-0125			PiColorlock Gold	2500/6250 assays
Pi结合树脂
501-0015			Pi Bind	5g
NTP琼脂糖
502-0002			ATP agarose Low (1-2 mmol/ml)	2ml
502-0005			ATP agarose Low (1-2 mmol/ml)	5ml
502-1000			ATP agarose combipack	1 pack
503-0002			ATP Bind	5 x 0.4g columns
505-0001			GTP agarose High (>6 mmol/ml)	2ml
505-0002			GTP agarose High (>6 mmol/ml)	5ml
506-0002			ADP agarose Low (1-2 mmol/ml)	2ml
506-0005			ADP agarose Low (1-2 mmol/ml)	5ml
510-0002			ATP agarose High (8-12 mmol/ml)	2ml
510-0005			ATP agarose High (8-12 mmol/ml)	5ml
511-0002			ADP agarose High (8-12 mmol/ml)	2ml
511-0005			ADP agarose High (8-12 mmol/ml)	5ml
520-0002			Control Resin	2ml
520-0005			Control Resin	5ml
NTPase酶检测试剂盒
601-0120			ATPase assay system	192 assays
601-0121			ATPase assay system	480 assays
601-0122			ATPase assay system	5 x 480 assays
602-0120			GTPase assay system	192 assays
602-0121			GTPase assay system	480 assays
602-0122			GTPase assay system	5 x 480 assays
其他产品
850-0005			PROTEIN A - HRP	0.5ml (3mg/ml conjugate)
851-0024			Protein A – Agarose	2ml packed vol
854-0005			PROTEIN A - ALKALINE PHOSPHATASE	0.5ml (3mg/ml conjugate)
857-0005			STREPTAVIDIN - HRP	0.5ml (3mg/ml conjugate)

858-0005	Protein A	1mg
858-0010	Protein A	5mg
859-0005	Goat anti-rabbit IgG - Agarose	2ml packed vol
860-0005	AbSelect Antibody Purification Kit	1 purification
860-0010	AbSelect Antibody Purification Kit	3 purifications
861-0005	STREPTAVIDIN – ALK. PHOSPHATASE	0.5ml (3mg/ml conjugate)
864-0005	STREPTAVIDIN - R-PHYCOERYTHRIN	0.5ml (3mg/ml conjugate)
865-0005	PROTEIN A - R-PHYCOERYTHRIN	0.5ml (3mg/ml conjugate)
888-0026	PROTEIN A - BIOTIN	0.5ml (3mg/ml conjugate)
888-0051	STREPTAVIDIN – FLUORESCEIN	0.5ml (3mg/ml conjugate)
888-0052	PROTEIN A – FLUORESCEIN	0.5ml (3mg/ml conjugate)
888-0077	STREPTAVIDIN - ALLOPHYCOCYANIN	0.5ml (3mg/ml conjugate)
888-0078	PROTEIN A - ALLOPHYCOCYANIN	0.5ml (3mg/ml conjugate)
888-0103	STREPTAVIDIN - R-PHYCOERYTHRIN	0.5ml (3mg/ml conjugate)
888-0129	STREPTAVIDIN– GLUCOSE OXIDASE	0.5ml (3mg/ml conjugate)
888-0130	PROTEIN A – GLUCOSE OXIDASE	0.5ml (3mg/ml conjugate)
889-0005	Biotinylated HRP	0.5ml (3mg/ml conjugate)
890-0005	Biotinylated PE	0.5ml (3mg/ml conjugate)