Sigma货号30693-1mg现货Chromeo™ P503上海睿安生物13611631389
属性
质量水平:100
荧光:λex 503nm;λem 627nm±10 nm in 0.1M bicarbonate buffer pH 8.3
储存温度:−20°C
说明
一般描述:Chromeo P503通过与伯胺结合后颜色从蓝色变为红色来标记蛋白质和肽。Chromeo P503显示弱荧光，量子产率为<1% in solution.After conjugation to a primary amine group, the label undergoes a shortwave spectral shift of>100nm，量子产率提高到50%。该特性可对伯胺、蛋白质和其他生物分子进行独特的检测。
应用:Chromeo P503用作荧光试剂，并用于标记分子(如蛋白质)内的伯胺基团。Chromeo P503标记的蛋白质可以在各种凝胶电泳和色谱应用中进行研究。
注意:为保证稳定性，冻干染料应避光保存于4℃。本产品自到货之日起保修6个月。
法律信息:Chromeo is a trademark of Active Motif Chromeon GmbH
同行评审论文
Attomole protein analysis by CIEF with LIF detection.
Lauren M Ramsay et al.
Electrophoresis, 30(2), 297-302 (2009-02-11)
We have coupled CIEF with an LIF detector that is based on a post-column sheath flow cuvette. We employed Chromeo P503 as a fluorogenic reagent to label proteins before analysis. This reagent reacts with the epsilon-amine of lysine residues, preserving
Reaction of fluorogenic reagents with proteins III. Spectroscopic and electrophoretic behavior of proteins labeled with Chromeo P503.
Emily H Turner et al.
Journal of chromatography. A, 1194(2), 253-256 (2008-05-17)
The spectroscopic and electrophoretic properties of proteins labeled with Chromeo P503 were investigated. Its photobleaching characteristics were determined by continually infusing Chromeo P503-labeled alpha-lactalbumin into a sheath-flow cuvette and monitored fluorescence as a function of laser power. The labeled protein
Protein labeling enhances aptamer selection by methods of kinetic capillary electrophoresis.
Stephanie de Jong et al.
Analytical chemistry, 83(16), 6330-6335 (2011-07-07)
Methods of kinetic capillary electrophoresis (KCE) facilitate highly ef_ficient selection of DNA aptamers for protein targets. The inability to detect native proteins at low concentrations in capillary electrophoresis creates, however, a significant obstacle for many important protein targets. Here we
Two-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection.
Jane A Dickerson et al.
Electrophoresis, 31(15), 2650-2654 (2010-07-07)
CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the
