化学发光EMSA试剂盒Gelshift™ Chemilumi

化学发光EMSA试剂盒Gelshift™ Chemiluminescent EMSA

Gelshift™ 化学发光EMSA

非放射性EMSA，简化了蛋白质-DNA绑定的筛选

The Gelshift Chemiluminescent EMSA Assay Kit provides a simple, non-radioactive assay to identify protein-DNA binding with proven reagents. In this electrophoretic mobility shift assay (EMSA), cell extracts or purified factors are incubated with biotin end-labeled probe containing the consensus binding site of interest. Samples in which the protein of interest bound the target DNA will migrate slower than DNA alone resulting in a "shift" of the labeled DNA band. For complete details, click the EMSA Method tab below.

Gelshift Chemiluminescent EMSA Assay Kit

The Gelshift Chemiluminescent EMSA Assay Kit includes all the reagents needed to study protein-DNA binding for your sample system. The kit also includes biotin-labeled and unlabeled control DNA and control nuclear extract. Click the Gel Shift tab below for data and more information; kit manuals can be downloaded under the Documents tab.

References

1. Fried, M. and Crothers, D.M. (1981) Nucleic Acids Res. 9: 6505-6525.
2. Revzin, A. (1989) BioTechniques 7: 346-354.
3. Hendrickson, W. (1985) BioTechniques 3: 198-207.

Contents & Storage

The Gelshift Chemiluminescent EMSA Kits contain 10X Binding Buffer, Biotin Control DNA, Unlabeled Control DNA, Control Nuclear Extract, Poly d(I-C), 50% Glycerol, 1% NP-40, 1 M KCl, 100 mM MgCl2, 200 mM EDTA pH 8.0 and 5X Loading Buffer, which are all stored at -20°C. Additionally, the kit includes Streptavidin HRP-conjugate, Chemiluminescent Reagent, Reaction Buffer, Blocking Buffer, 4X Wash Buffer and Substrate Equilibration Buffer which should be stored at 4°C.