Sigma货号I9516-25ml异丙醇[2-丙醇(2-Propanol)]上海睿安生物13611631389

 

Sigma货号I9516-4✕25ml异丙醇[2-丙醇(2-Propanol)]上海睿安生物13611631389

Sigma货号I9516-500ml异丙醇[2-丙醇(2-Propanol)]上海睿安生物13611631389

Sigma货号I9516-1L异丙醇[2-丙醇(2-Propanol)]上海睿安生物13611631389

2-丙醇

≥99.5%,for molecular biology,suitable for RNA extraction,DNA extraction,BioReagent

别名:IPA,仲丙醇,异丙醇

说明

一般描述:异丙醇是一种透明、无色、极性的有机溶剂，常用于化学和分子生物学实验室。它可溶解一系列的化物品并快速蒸发。本品(2-丙醇)为分子生物学级，适用于核酸沉淀。

异丙醇使用方案:

• 从哺乳动物组织沉淀DNA，从DNA样品中提取溴化乙锭，以及从细胞和组织中分离RNA。
• 蛋白质结晶。
• 从生物基质中分离类维生素A，进行HPLC和HPLC-MS分析。

应用

2-丙醇已用于:

• DNA和RNA萃取
• 测定羟脯氨酸水平(《The Merck Index》第12版)
• MTT测定检验细胞活力
• 芯片杂交

适用于使用标准实验流程用于DNA沉淀。还适合作为针对分子生物学应用所制备溶液中的一种溶剂。

同行评审论文(部分文献)

Crystal structure of cyanovirin-N, a potent HIV-inactivating protein, shows unexpected domain swapping.F Yang et al.Journal of molecular biology, 288(3), 403-412 (1999-05-18)The crystal structure of cyanovirin-N (CV-N), a protein with potent antiviral activity, was solved at 1.5 A resolution by molecular replacement using as the search model the solution structure previously determined by NMR. The crystals belong to the space groupSambrook, J., et al.Molecular Cloning: A Laboratory Manual, E-E (1989)High responders to resistance exercise training demonstrate differential regulation of skeletal muscle microRNA expression.Peter K Davidsen et al.Journal of applied physiology (Bethesda, Md. : 1985), 110(2), 309-317 (2010-10-30)MicroRNAs (miRNA), small noncoding RNA molecules, may regulate protein synthesis, while resistance exercise training (RT) is an efficient strategy for stimulating muscle protein synthesis in vivo. However, RT increases muscle mass, with a very wide range of effectiveness in humans.Expression of genes involved in neurogenesis, and neuronal precursor cell proliferation and development: Novel pathways of human ovarian granulosa cell differentiation and transdifferentiation capability in vitro.Maciej Brązert et al.Molecular medicine reports, 21(4), 1749-1760 (2020-04-23)The process of neural tissue formation is associated primarily with the course of neurogenesis during embryonic life. The source of neural‑like cells is stem cells, which, under the influence of appropriate differentiating factors, may differentiate/transdifferentiate towards a neural‑like lineage. TheSynthesis of a novel, recyclable, solid-phase acylating reagent.Robert B Nicewonger et al.Bioorganic & medicinal chemistry letters, 12(14), 1799-1802 (2002-06-28)In this paper, we describe the synthesis of N-(6-cyano-1,3-dimethyl-2,4-dioxo-5-substituted-1,3-dihydropyridino[2,3-d] pyrimidin-7-yl)imides 1. We will show the synthesis of 1 using both conventional heating and microwave techniques. In addition, the imide was attached to polystyrene and this immobilized imide was equally as