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PurKine™ 组氨酸标签蛋白纯化试剂盒（Ni-NTA树脂）KTP2001
◆ 产品定位与应用场景
核心级蛋白纯化工具，专为组氨酸标签融合蛋白设计，适用于细胞器提取后的靶蛋白捕获及包涵体变性纯化双重场景。兼容天然/变性条件，满足结构生物学及体外功能研究需求。
◆ 技术参数矩阵
- 树脂载量：≥50 mg 6xHis-融合蛋白/mL树脂
- 循环次数：≥5次性能稳定（建议0.5 M NaOH再生）
- 缓冲体系：预配10X PBS + 梯度咪唑溶液
◆ 多形态交付方案
| 规格 | 价格（CNY） | 库存状态 |
|---|---|---|
| 1 mL | 769 | 期货（2日内交付） |
| 1 mL×5 | 1916 | 当日发货（库存≥200套） |
◆ 操作体系兼容性
适配IMAC标准工作流程：
镍柱与IDA/NTA配体协同作用，支持：
• 天然条件（pH 7.4-8.5）温和洗脱
• 变性条件（4-8 M尿素/盐酸胍）强力溶解
▣ 冷链管理声明
- 储运温度：2-8°C恒温（避光防冻）
- 有效期：生产批号+12个月
- 运输保障：医用级蓝冰+独立冷链箱
! 重要使用声明
本产品仅限科研用途，不符合GMP生产规范。建议配合His标签验证试剂（Western Blot/HPLC）进行纯度验证，具体应用场景请自行确认专利合规性。
技术备注：基于IMAC原理的镍柱介质，建议结合Co2+螯合介质（货号KTP2002系列）进行交叉验证。
```注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
PurKine™ His-Tagged Protein Purification Kit (Ni-NTA Resin) KTP2001
◆ Product Positioning & Applications
Core-grade protein purification tool specifically designed for His-tagged fusion proteins, suitable for both target protein capture post-organelle extraction and inclusion body denaturing purification. Compatible with native/denaturing conditions, meeting structural biology and in vitro functional study requirements.
◆ Technical Parameter Matrix
- Resin Capacity: ≥50 mg 6xHis-fusion protein/mL resin
- Reusability: ≥5 cycles with stable performance (recommended regeneration with 0.5 M NaOH)
- Buffer System: Pre-formulated 10X PBS + imidazole gradient solutions
◆ Multi-format Delivery Options
| Specification | Price (CNY) | Inventory Status |
|---|---|---|
| 1 mL | 769 | Futures (delivery within 2 days) |
| 1 mL×5 | 1916 | Same-day shipping (inventory ≥200 sets) |
◆ Operational System Compatibility
Compatible with IMAC standard workflows:
Nickel columns synergize with IDA/NTA ligands, supporting:
• Gentle elution under native conditions (pH 7.4-8.5)
• Strong solubilization under denaturing conditions (4-8 M urea/guanidine HCl)
▣ Cold Chain Management Statement
- Storage/Transport Temperature: 2-8°C constant (light-sensitive, freeze-protected)
- Shelf Life: 12 months from production batch
- Transport Assurance: Medical-grade blue ice + independent cold chain box
! Critical Usage Statement
For research use only, non-GMP compliant. Recommended to pair with His-tag validation reagents (Western Blot/HPLC) for purity verification. Users must independently confirm patent compliance for specific applications.
Technical Note: IMAC-based nickel column media should be cross-validated with Co2+-chelating media (Catalog No. KTP2002 series).
Note: The above information may be subject to changes due to product optimization. For updated details, please consult Yakoyin Company.
