Prescission Protease(PP酶)

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  • 486.11/

上海觅拓生物科技有限公司
18616635231 00000000
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  • Brand  BM
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  • Main Business:细胞,载体,试剂,技术服务,实验室耗材
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Last Updated 2025-08-12 15:30

Prescission Protease(PP酶)

储运温度:长期储存于-80℃,可保存2年;或小量分装后保存于-20℃,可保存6个月,避免反复冻融。10×Cleavage Buffer 置于-20℃ 保存。

 

产品介绍:

         Prescission Protease(简称PSP)是一种由人鼻病毒14型的3C蛋白酶和GST组成的融合蛋白。这种蛋白酶特异识别短肽Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro中的Gln和Gly残基而进行切割,底物的识别和切割不仅依赖于融合蛋白的一级结构还依赖于融合蛋白的二级和三级结构。它可以特异性的将pGEX-6P系列等载体表达出的带有酶底物识别多肽序列融合蛋白的GST标签进行分离。本蛋白来源为大肠杆菌重组表达。

 

酶活性单位定义:在5℃条件下反应 16小时,能够切割100 µg的GST标签的融合蛋白达90%以上所需的酶量定义为一个活性单位。

 

PSP贮存液:

      25mM Tris-HCl,pH 8.0、150mM NaCl、1mM EDTA、5mM DTT、50%Glycerin

     10×Cleavage Buffer:

     500mM Tris-HCl,pH 7.0、1.5M NaCl、10mM EDTA、10mM DTT

Company Name 上海觅拓生物科技有限公司
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Business Scope 细胞,载体,试剂,技术服务,实验室耗材
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Medicine Platform
 
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