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Zeocin™ Selection Antibiotic (100 mg/mL)（细胞培养检测，无菌级）
描述
博来霉素报价
100 mg⁄ml
Regulatory Statement:
增光霉素（博来霉素）别名：争光霉素Zeocin
Zeocin®|选择抗生素：内毒素测试，无菌试剂
| 商品名称 | 工作浓度 | 稳定性 | 浓度 | 规格 | 产品编号 |
| Blasticidin | Cells: 1-10 μg/ml E. coli: 25-100 μg/ml | >1 year at -20°C >1 year at 4°C 2 weeks at 20-25°C | 10 mg/ml | 100 mg 500 mg | ant-bl-1 ant-bl-5 |
| Zeocin™ | Cells: 50-300 μg/ml E. coli: 25 μg/ml | >1 year at -20°C >1 year at 4°C 1 month at 20-25°C | 100 mg/ml | 1 g 5 g | ant-zn-1 ant-zn-5 |
纯度：>95%
Zeocin™ – 獒合钢糖肽类抗生素，通过嵌入DNA中，使DNA断裂而导致细胞死亡。对于大多数耗氧细胞都是有作用的。广泛用于细菌、真核微生物、植物和动物细胞的筛选。抗性基因Sh ble。 纯度：>90%
InvivoGen高品质基因筛选抗生素
▲ 细胞培养即用型
可提供直接用于细胞培养，无需繁琐步骤
▲ 内毒素测试
产品均通过严格的内毒素检测，避免实验结果偏差
▲ 严格的质控
严格的生产及质控标准，对批次间差距有严格控制
▲ 灵活的包装
InvivoGen是全球唯一的Zeocin®生产商。
Zeocin®是一种由链霉菌CL990生产的铜螯合糖肽抗生素维霉素D1的配方。Zeocin®通过插入DNA并将其切割而导致细胞死亡。
Zeocin®的作用对大多数需氧细胞有效。因此，Zeocin®是一种流行且有效的抗生素，用于在多种细胞类型（细菌、真核微生物、植物和动物细胞）中选择携带Sh ble基因的载体。
对Zeocin®的耐药性由来自印度斯坦链球菌的稳定基因产物赋予，该基因产物在与抗生素结合时使Zeocin®失活[1-3]。
Sh ble是一个370bp大小的小基因，携带在许多InvivoGen载体中。
通常，哺乳动物细胞对50-400µg/ml的Zeocin®浓度敏感，对25µg/ml的细菌敏感。规格
产品浓度：溶液中100 mg/ml
CAS编号：11006-33-0
质量控制：每个批次都经过彻底测试，以确保没有批次间的差异。
内毒素水平：<1 EU/mg
理化特性：HPLC、pH、外观
细胞培养测试：在Zeocin®敏感和Zeocin®抗性哺乳动物细胞系中验证效力
微量污染物的非细胞毒性：在Zeocin®抗性细胞中确认无长期效应
Selection antibiotic: cell culture tested, sterile reagent
InvivoGen is the sole worldwide producer of Zeocin™, a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).
Resistance to Zeocin™ is conferred by the Sh ble gene product, which inactivates Zeocin™ by binding to the antibiotic. Sh ble is a small gene, only 370bp in size, carried in a number of InvivoGen’s vectors.
Zeocin™ is a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it. The action of Zeocin™ is effective on most aerobic cells.
Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.
Product concentration: 100 mg/ml in solution
CAS number: 11006-33-0
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Endotoxin level: < 1 EU/mg
Physicochemical characterization: HPLC, pH, appearance
Cell-culture tested: potency validated in Zeocin™-sensitive and Zeocin™-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in Zeocin™-resistant cells
Zeocin™ is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer. This product is available in three pack sizes:
- ant-zn-1: 10 x 1 ml (1 g)
- ant-zn-5: 50 x 1 ml (5 g)
- ant-zn-5b: 1 x 50 ml (5 g)
Zeocin™ is also supplied as a blue powder:
- ant-zn-1p: 1 x 1 g
Recent articles using InvivoGen Zeocin™
- 2017 - Nature., 543(7646):568-572.
DND1 maintains germline stem cells via recruitment of the CCR4–NOT complex to target mRNAs.
Yamaji M. et al. - 2017 - Cell., 168(3):503-516.
Pathogen-Mediated Inhibition of Anorexia Promotes Host Survival and Transmission.
Rao S. et al. - 2017 - Nat Commun., 8:14391.
IFI16 is required for DNA sensing in human macrophages by promoting production and function of cGAMP.
Jønsson KL. et al. - 2017 - Sci Rep., 7:44328.
Plectin is a novel regulator for apical extrusion of RasV12-transformed cells
Kadeer A. et al. - 2017 - PNAS, [Epub ahead of print]
Rational design of adjuvants targeting the C-type lectin Mincle.
Decout A. et al.
For Research Use Only. Not for use in diagnostic procedures.
上海研卉生物科技有限公司 ，是一家专注于生命科学和生物技术领域的高科技企业，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司主营业务： 试剂盒：酶联免疫试剂盒、免疫印迹试剂盒、免疫组化试剂盒、细胞凋亡检测试剂盒、分子生物学试剂盒。 细胞：原装进口细胞、国产肿瘤细胞、国产正常细胞。 耗材：细胞培养耗材、普通实验耗材。 生化试剂：原装进口生化试剂、进口分装生化试剂、国产生化试剂。 抗体：免疫组化、免疫荧光、流式细胞检测、免疫细胞化学。
