Zymo Research - D6005 - Quick-

Name ：Quick-DNA Fungal/Bacterial Miniprep Kit

Cat # ：D6005

Size ： 50 Preps

Brand：Zymo Research

 

_d6005_quick-dna_fungal-bacterial_miniprep_kit.pdf

 

HIGHLIGHTS

Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.

Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.

Simple: Fastest workflow (≤ 20 minutes).

 

DESCRIPTION

The Quick-DNA Fungal/Bacterial Miniprep Kit is designed for the simple and rapid isolation of DNA
from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe,
as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in
minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art,
ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA
that is ideal for downstream molecular-based applications including PCR, array, etc.

 

TECHNICAL SRECIFICATIONS

Applicable For

All sensitive downstream applications such as qPCR and Next-Generation Sequencing.

Elution Volume

≥ 35 µl

Equipment

Microcentrifuge, vortex, cell disruptor/pulverizer

Processing Time

≤ 15 minutes

Processing Volume

≤100 mg fungi or bacteria (wet weight), 109 bacterial cells, 108 yeast cells, or 107 mammalian cells

Purity

Typical A260/A280 & A260/A230 ≥ 1.8

Sample Source

Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can
also be sampled.

Size Range

Capable of recovering genomic DNA sized fragments from up to and above 40 kb. Typical fragment
sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered

Type

Total DNA

Yield

≤ 25 µg total DNA

Supplemental Info

Purpose of Genomic DNA Extraction & Tips For Success