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DNA Clean & Concentrator-5
Cat # Name Size
D4004 DNA Clean & Concentrator-5 (Uncapped) 200 Preps
D4003 DNA Clean & Concentrator-5 (Uncapped) 50 Preps
D4014 DNA Clean & Concentrator-5 (Capped) 200 Preps
D4013 DNA Clean & Concentrator-5 (Capped) 50 Preps
D4003T DNA Clean & Concentrator-5 (Uncapped) 10 Preps
Brand：Zymo Research
_d4003t_d4003_d4004_d4013_d4014_dna_clean_concentrator_-5.pdf
HIGHLIGHTS
Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash
residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or
TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion,
RNA transcription, radiolabeling, arrays, etc.
DESCRIPTION
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg
DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling
reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA
polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as
free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is
suitable for PCR, arrays, ligation, sequencing, etc.
TECHNICAL SRECIFICATIONS
Detergent Tolerance
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume
≥ 6 µl
Equipment
Microcentrifuge
Purity
A260/A280 > 1.8
Sample Source
DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling
reactions, etc.
Size Range
50 bp to 23 kb
Yield
≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb
to 23 kb the recovery is 50-70%.
