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末端脱氧核苷酸转移酶（TdT） - PRP3002
核心工具酶，精准调控细胞状态研究
◆ 产品概述
重组牛源末端脱氧核苷酸转移酶（TdT）采用严格质控工艺制备，无宿主蛋白残留，经SDS-PAGE验证纯度>90%，分子量58.3 kDa。适用于DNA 3'末端标记、平末端/粘性末端延伸等分子生物学实验。
◆ 关键参数
- 基因来源：牛TdT (UniProt ID: P06526)
- 蛋白序列：Mer1-Ala520全长520aa
- 活性单位：≥2 mg/ml（出厂标定浓度）
- 缓冲体系：50%甘油储存液，即用型配方
◆ 试剂盒组分
- TdT蛋白复溶液（含BSA稳定剂）
- 5X Reaction Buffer（优化反应体系）
◆ 技术优势
- 非模板依赖性核苷酸聚合，支持dNTP/ddNTP多种底物
- 严格验证3'突出端/平端/凹陷端修饰效率
- 即用型溶液免除繁琐复溶步骤
◆ 物流与存储
- 运输方式：蓝冰低温运输（-20℃恒温）
- 长期保存：-20℃分装避光，避免>3次冻融循环
- 工作液保存：稀释后4℃稳定≥72小时
◆ 订购信息
| 规格 | 价格（CNY） | 货期 |
|---|---|---|
| 40 μg | 498 | 现货 |
| 100 μg | 699 | 现货 |
| 1 mg | 3598 | 定制（7-10工作日） |
◆ 重要提示
实验前务必离心5秒以收集管壁附着蛋白，推荐使用配套缓冲液进行反应体系配置。本产品仅限科研使用，不适用于GMP生产或体外诊断。
```注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Terminal Deoxynucleotidyl Transferase (TdT) - PRP3002
Core Tool Enzyme for Precise Regulation of Cellular State Research
◆ Product Overview
Recombinant bovine-derived Terminal Deoxynucleotidyl Transferase (TdT) is prepared under stringent quality control processes, free of host protein residues, with purity >90% verified by SDS-PAGE and a molecular weight of 58.3 kDa. Suitable for molecular biology experiments such as DNA 3' end labeling, blunt/sticky end extension.
◆ Key Parameters
- Gene Source: Bovine TdT (UniProt ID: P06526)
- Protein Sequence: Full-length 520aa from Mer1-Ala520
- Activity Unit: ≥2 mg/ml (factory-calibrated concentration)
- Buffer System: 50% glycerol storage solution, ready-to-use formulation
◆ Kit Components
- TdT protein reconstitution solution (contains BSA stabilizer)
- 5X Reaction Buffer (optimized reaction system)
◆ Technical Advantages
- Template-independent nucleotide polymerization, supports various substrates including dNTP/ddNTP
- Rigorously validated modification efficiency for 3' overhangs/blunt ends/recessed ends
- Ready-to-use solution eliminates tedious reconstitution steps
◆ Logistics & Storage
- Shipping Method: Blue ice cold chain transport (-20°C constant temperature)
- Long-term Storage: -20°C aliquoted and protected from light, avoid >3 freeze-thaw cycles
- Working Solution Storage: Stable at 4°C for ≥72 hours after dilution
◆ Ordering Information
| Specification | Price (CNY) | Lead Time |
|---|---|---|
| 40 μg | 498 | In stock |
| 100 μg | 699 | In stock |
| 1 mg | 3598 | Custom (7-10 working days) |
◆ Important Notes
Centrifuge for 5 seconds before use to collect protein adhered to tube walls. It is recommended to use the provided buffer for reaction system setup. This product is for research use only, not suitable for GMP production or in vitro diagnostics.
Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult Yakeyin Company.
