5637(人膀胱癌细胞)

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上海经科化学科技有限公司
13524423207 021-54997581
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  • Main Business:生化试剂,标准品,培养基,分子生物学产品
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Last Updated 2025-08-12 15:39

5637(人膀胱癌细胞)

产品信息
名称293 [HEK-293] (人胚肾细胞) (STR鉴定正确)
别称Hek293; HEK-293; HEK 293; HEK:293; 293; 293 HEK; Human Embryonic Kidney 293
种属
生长特性贴壁细胞
细胞形态上皮细胞样
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养方案A(默认)生长培养基:MEM(ATCC改良)+10% FBS+1% P/S培养条件:气相:空气,95%;CO2,5%, 温度:37℃
推荐传代比例1:3-1:4
推荐换液频率2-3次/周
注意事项该细胞贴壁松散,操作时请尽量轻柔;换液时需预热培养基;收货如有大块脱落的细胞团,为正常现象,请按照收货注意事项处理。
参考资料(来源文献)
背景描述293 [HEK-293]细胞是剪切过的人腺病毒5(Ad5)转染的人胚肾细胞形成的永生化细胞,293 [HEK-293]细胞包含并表达转染的Ad5基因。早期报道中指出,293 [HEK-293]细胞基因组中含有腺病毒5(Ad5)基因组的左侧端和右侧端的DNA,但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现,Ad5的1-4344位线性核苷酸整合入293 [HEK-293]细胞19号染色体(19q13.2)。293 [HEK-293]细胞为人类腺病毒载体扩增的宿主,可表达异常的玻连蛋白的细胞表面受体,由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。
年龄(性别)胎儿
组织来源肾;以腺病毒5DNA进行转化
细胞类型转化细胞系
生物安全等级BSL-2
倍增时间~24-30 hours
致瘤性Yes, forms tumors in nude mice.
受体表达情况vitronectin
保藏机构ATCC; CRL-1573 ATCC: PTA-4488 DSMZ: ACC-305 ECACC: 85120602
STR鉴定图
  • STR位点信息
    AmelogeninX
    CSF1PO12
    D2S133819
    D3S135815,17
    D5S8188
    D7S82011,12
    D8S117912,14
    D13S31712,14
    D16S5399,13
    D18S5117,18
    D19S43315,18
    D21S1128,30.2
    FGA23
    PentaD9,10
    PentaE7,15
    TH017,9.3
    TPOX11
    vWA16,19
    D6S104311
    D12S39119,21
    D2S44111,15
  • STR鉴定图
Company Name 上海经科化学科技有限公司
Location
Business Scope 生化试剂,标准品,培养基,分子生物学产品
Contact Information
Medicine Platform
 
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