爆款Roche货号04942078001分散酶II上海睿安生

爆款现货Roche货号04942078001分散酶II(中性蛋白酶，II级)[规格包装(5╳1g)/盒](目录价¥8432.79元/盒╳13611631389上海睿安生物:Roche代理说明一般描述:分散酶是一种中性、快速反应、温和的蛋白酶，能将表皮从真皮中完整分离。它还能将培养的上皮细胞从基质中完整分离出来。其可分裂基底膜区，使上皮细胞保持完整。 特异性:分散酶是一种非特异性的金属蛋白酶。 应用:分散酶®用于从多种不同的组织和器官制备细胞。该酶已经证实是一种快速、有效但温和破坏组织胞外基质的制剂，可释放用于细胞培养的单细胞(原代细胞培养)或收集培养细胞以转至新底物中(继代培养)。此外，它还可用来防止细胞悬浮培养过程中形成不必要的团块。特点和优势:Rapid，effective，yet gentle agent that liberates cells with minimal cell damage Maintains cell membrane integrity Non-mammalian source-free of mycoplasma and animal virus contamination Extremely stable to influences of temperature，pH，and interference by serum components Easily inactivated by chelating agents or by dilution Delivers higher activity and convenience 单位定义:一单位是指在pH 7.5、+37°C反应条件下，在1分钟内释放出对应1μmol(181μg)酪氨酸的Folin-阳性的氨基酸和肽类所需的酶量。Roche分散酶®II单位与日本文献中引用的分散酶单位(其中分散酶®浓度为1000到2000单位/ml的情况不常见)的实际比较结果表明，Roche的1单位分散酶大致相当于日本的600单位。Roche Applied Science的1单位®相当于181蛋白酶单位(PU)，其中PU为在pH 7.5和+37°C条件下，在1分钟内释放相当于1μg酪氨酸的氨基酸所需的酶量。 制备说明:激活剂:最佳Ca2+浓度为2mM。酶制剂含有维持最佳活性所需的足量Ca2+。抑制剂:EDTA、EGTA、Hg2+、其他重金属。分散酶不受血清抑制。工作浓度:0.6至2.4U/ml工作溶液:储备溶液和工作溶液制备: 制备10mg/ml的储备溶液，将分散酶®II冻干粉溶于HEPES-缓冲盐溶液中(50mM HEPES/KOH pH 7.4，150mM NaCl)。制备工作溶液，将上述储备溶液用分离细胞培养基稀释至终浓度为0.6到2.4U/ml。请注意，建议浓度不要高于2.4U/ml。为实现最佳结果，用0.22μm滤膜过滤工作溶液。储存条件(工作溶液):-15至-25°C复溶的储备溶液在2至8℃下可稳定保存2周。如需保存2个月以上，储备溶液需分装冻存。避免反复冻融! 采用PBS稀释的工作溶液在2至8℃下可稳定保存3天。储存及稳定性:2至8°C储存。(干燥储存!)其他说明:仅用于生命科学研究。不可用于诊断。

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SARS-CoV-2 Receptor ACE2 Is Enriched in a Subpopulation of Mouse Tongue Epithelial Cells in Nongustatory Papillae but Not in Taste Buds or Embryonic Oral Epithelium.Zhonghou Wang et al.ACS pharmacology & translational science, 3(4), 749-758 (2020-08-22)As a result of the COVID-19 pandemic, evidence revealed that SARS-CoV-2 infection caused taste loss at a rate higher than that of influenza. ACE2, the entry receptor of SARS-CoV-2, has been identified in the oral epithelium; however, it is unclearThe adaptor protein CIKS/ACT1 is necessary for collagen-induced arthritis, and it contributes to the production of collagen-specific antibody.Prapaporn Pisitkun et al.Arthritis and rheumatism, 62(11), 3334-3344 (2010-07-28)CIKS/ACT1 is an adaptor molecule that is necessary for signaling by members of the interleukin-17 cytokine family. The aim of this study was to determine whether this adaptor is required for the initiation of collagen-induced arthritis (CIA). If it isComparative Molecular Analysis of Cancer Behavior Cultured In Vitro, In Vivo, and Ex Vivo.Nicholas R Hum et al.Cancers, 12(3) (2020-03-19)Current pre-clinical models of cancer fail to recapitulate the cancer cell behavior in primary tumors primarily because of the lack of a deeper understanding of the effects that the microenvironment has on cancer cell phenotype. Transcriptomic profiling of 4T1 murineIL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells.Johanna Tukler Henriksson et al.Investigative ophthalmology & visual science, 56(8), 4186-4197 (2015-07-02)To investigate the effects of IL-13 on goblet cell proliferation, differentiation, and expression of mucin and immunomodulatory genes. Explants were excised from the conjunctiva of young C57BL/6 mice. Cultures received 200 μL per week of either Keratinocyte media (KSFM) orAutologous fibroblasts induce fibrosis of the nucleus pulposus to maintain the stability of degenerative intervertebral discs.Chen Chen et al.Bone research, 8, 7-7 (2020-03-05)Lumbar degenerative disc diseases cause low back pain (LBP). The maintenance of the height and stability of the intervertebral disc (IVD) space is an effective treatment for LBP. The following study evaluated the effects of fibroblast injection on intervertebral disc 一、╳※试剂※╳:常规血清优势供应:VivaCell货号C04001-500血清、Gemini货号900-108血清、Gemini货号100-512血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清培养基(包括无血清)、冻存液(包括无血清)，包括:友康YoconMerck(Millipore/Sigma/Roche/KaPa)正式授权代理、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen饲料类:Research Diets、Dyets二、╳※耗材※╳:    洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶    Bioplastics    Thermo Fisher Scientific的很多产品线，包括旗下的:    一、Nunc耗材    二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)    三、Nalgene耗材仪器    四、FisherBrand耗材仪器(含各类盖玻片载玻片等)    五、Lab_Serv耗材仪器    六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)    七、Abgene耗材畅销款:我们是常备有大量现货的※╳√√✓✔√✓✔√✓✔上海睿安生物科技有限公司成立于2009年，专注于为生命科学和生物技术领域的客户提供优质的产品与服务。代理了多家欧美知名品牌，如美国Thermo Fisher耗材(包括旗下Nunc、QSP、Nalgene、FisherBrand、Lab_Serv耗材仪器、Finnpipette移液器、Abgene耗材，等等)、默克Merck-Millipore/Sigma/Roche/KaPa、Zymo Research、洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理、Eppendorf、友康Yocon、Research Diets高脂饲料，IBL、BioVendor、Mercodia、ShiBayaji代谢类产品、Axygen-Corning长期大量耗材备货、BioCaot畅销款长期备货，VivaCell血清、Gemini血清、Gibco血清，BI血清现货、ScienCell血清、Sigma血清、Moregate血清、Cegrogen血清、Pierce发光试剂、Fermentas、Invitrogen、Abcam、CST、BD、SAB、R&D systems、Beckman、Qiagen、BD Pharmingen、Biolegend、Jackson、Miltenyi、MP Biomedicals、Biotium更好的荧光试剂产品等品牌。睿安生物始终将“提供更好的产品和服务”作为企业发展的目标和努力的方向，并将通过优质的售前与售后服务，始终如一地为客户提供高质量的产品和服务，我们也希望能得到相关领域中更多老师和同仁们的指导和鞭策，为共创优秀品牌而努力奋斗！询订╳直线13611631389微信号