Phusion® 超保真 DNA 聚合酶#M0530S

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北京智杰方远科技有限公司
15810200961 010-82897201
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Last Updated 2025-08-12 15:47

Phusion® 超保真 DNA 聚合酶#M0530S

特性:
超高保真性
快速 PCR
常规 PCR
高产量
长片段扩增
高通量
复杂模板的扩增

概述:PhusionR 超保真 DNA 聚合酶性能卓越,适用于多种不同的模板。其独特的结构包括一个类似 Pyrococcus 的酶和一个掺入率增强结构域,二者融合在一起。这种组合提升了聚合反应的保真性和速度,以及酶的整体性能。其错配率比 Taq 低50 倍,比 Pfu 低 6 倍,这使得该酶成为目前市场上保真度最高的热稳定聚合酶。可供选择的试剂包括单独包装的预混液和试剂盒,以及针对复杂模板的反应缓冲液。Phusion DNA 聚合酶是克隆的最佳用酶,其为 PCR 反应建立了新的标准。

热失活:无

Phusion 超保真 PCR 试剂盒组成:
5X Phusion GC 和 HF 缓冲液
Phusion 超保真 DNA 聚合酶 (2,000 units/ml)
1.3 kb 和 10 kb 引物 (每种 4 μM)
λ 对照模板 (0.5 ng/μl)
dNTP 混合液 (每种 10 mM)
DMSO (100%)
DNA 标准 (λ DNA HindIII + φX174 DNA HaeIII 消化) (50 ng/μl)
MgCl2 溶液 (50 mM)
Company Name 北京智杰方远科技有限公司
Location
Business Scope 进口胎牛血清,美国GIBCO,德国PAN,以色列BI,澳大利亚Ausgene-X,德国Cegrogen,美国GEMINI,美国Hyclone,MP Bio,日本同仁,TAKARA,Biolog微生物鉴定板,Borer清洗产品,台湾旭基
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