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Highlights:
- Extreme short working time: only one minute stripping to remove primary and secondary antibodies
- Harmless formula: free of reducing agents, toxic compounds and odors
Order Information:
| Cat. No. | Product Name | Description |
| SP01-500 | StripPRO™ 1 Min Stripping Buffer | 500 mL Solution X 1 |
| SP05-100 | StripPRO™ 1 Min Stripping Buffer (5X) | 100 mL Solution X 1 |
Product Detail:
Figure 1. StripPRO™ 1 Min Stripping Buffer removes antibodies from blot in only 1 min
Huh7 cell lysate was probed for ACC (280kD). Blots were then stripped with either StripPRO™ (1min) or brand T (15 mins). The blots were then re-blocked and reprobed for GAPDH (36kD) and detected with LumiFlash™ Ultima Chemiluminescent Substrate.
Figure 2. Stripping and reprobing blot for different molecular weight targets with StripPRO™
1/2 fold serial dilution of Hela cell lysate (start from 20ug/well) were separated by SDS-PAGE. The proteins were transferred to 0.45um PVDF membranes and the blocked with BlockPRO™ 1 Min Protein-Free Blocking buffer (BM01) and analyzed by Wetsern blot using LumiFlash™ Ultima Chemiluminescent substrate, HRP system (LF08) and the Chemlux SPX-600V (2x2 binning). The first target (1st detection) was detected at 1:1,000 by probing with Anti-ACC monoclonal antibody (#3676, Cell Signaling Technology). And then the blot was stripped in 1 Min Stripping Buffer (SP01) for 1 minute at room temperature, washed in 1X TBS Tween-20, incubated with substrate and imaged to check for stripping efficiency (1st strip test) The second target (2nd detection) was detected at 1:1000 by reblocking and probing with Anti-p38 monoclonal antibody (ab31828, Abcam). The blot was stripped again (2nd strip test) and then the third target (3rd detection) was detected at 1:10,000 by reblocking and probing with Anti-GAPDH antibody (ab8245, Abcam). All of the secondary antibodies was proved at 1:10,000 (#115-035-003, 3115-035-144, Jackson ImmunoResearch)
Figure 3. Demonstration of phosphorylated protein and original type in one blot by using StripPRO™ 1 Min Stripping Buffer
Hela cell lysate was extracted with RIPA Cell Lysis Buffer (RP05) added with PhosSTOP (Roche). 1/2 fold serial dilution of Hela Cell lysate (start from 20ug/well) were separated by SDS-PAGE. The two membranes were probed for p-ACC and GAPDH respectively and detected with LumiFlash™ Ultima Chemiluminescent substrate, HRP system (LF08). Blots were stripped in StripPRO™ 1 Min Stripping Buffer (SP01) for 1 minute at room temperature. The blots were reblocked and reprobed for ACC and GAPDH respectively.
Figure 4. Stripping efficiency of StripPRO™ 1 Min Stripping Buffer in two common transfer membranes
1/2 fold serial dilution of Hela Cell lysate (start from 20ug/well) were separated by SDS-PAGE. Following protein transferred to nitrocellulose (NC) paper and PVDF, the two membranes were probed for ACC and detected with LumiFlash™ Ultima Chemiluminescent substrate, HRP system (LF08). Blots were stripped in StripPRO™ 1 Min Stripping Buffer (SP01) for 1 minute at room temperature. The blots were reblocked and reprobed for GAPDH.
