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Human TNF-alpha protein
产品名：Human TNF-alpha protein
货号：orb257883
产品中文名：Human TNF-alpha 蛋白
| Catalog Number | orb257883 |
| Category | Proteins |
| Description | Human TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1). |
| Reactivity | Human |
| Tag | Native |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 17.4 kDa |
| Target | TNF-alpha |
| UniProt ID | P01375 |
| Source | Human TNF-alpha, premium grade (orb257883) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Val 77 - Leu 233 |
| Endotoxins | 0.01 EU per ug |
| NCBI | NP_000585.2 |
| Buffer/Preservatives | PBS, pH7.4 |
| Note | For research use only |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human TNF-alpha protein
Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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