Roche货号10843555001潮霉素B(Hygromycin B)上海睿安生物13611631389
潮霉素B
from Streptomyces hygroscopicus
别名:潮霉素B来源于吸水链霉菌
说明
一般描述:
- 分子式:C20H37N3O13
- LD50:小鼠6mg/kg(静脉注射);豚鼠13mg/kg(腹腔注射);大鼠63mg/kg(腹腔注射)
- 分子量:Mr=527.5
- 工作浓度:细胞培养中50-1000μg/ml。用于选择哺乳动物细胞的常用浓度是200μg/ml。但是，最佳浓度必须通过实验确定，因为它可能会因使用的细胞类型而异。
应用:潮霉素B是一种氨基糖苷抗生素，可抑制原核生物和真核生物中的蛋白质合成。它被用于选择和维持可以稳定转染大肠杆菌潮霉素抗性基因(hyg或hph)的真核细胞的表型。
生化/生理作用
作用机制:本品通过诱导原核生物中m-RNA模板的误读，抑制蛋白质合成，具有抑制翻译的作用。抗菌谱:潮霉素B对细菌、真菌和高等真核细胞有抑制作用。
质量
纯度:>80%(HPLC)单位定义
单位试验:Unit/mg我们没有这些信息。
外形:溶液，50mg/ml，溶于PBS(磷酸盐缓冲生理盐水)中，已通过0.2μm孔径滤膜过滤。
制备说明
工作浓度:在细胞培养中用于选择抗性细胞的推荐浓度是1000μg/ml。 用于选择哺乳动物细胞的常用浓度是200μg/ml。 但是，最佳浓度必须通过实验确定，因为可能会因使用的细胞类型而异。储存及稳定性:2至8°C下储存。(溶液)
其他说明:仅用于生命科学研究。不用于诊断操作。溶液，50mg/ml，溶于PBS(磷酸盐缓冲生理盐水)中，已通过0.2μm孔径滤膜过滤。
同行评审论文(部分文献)
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订购信息:
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| 11685597910 | Biotin RNA Labeling Mix 10x 生物素核酸标记 | 40 µl (20 reactions) |
| 11277073910 | DIG RNA Labeling Mixture, 10x | 40 µl (20 reactions) |
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| 6366244001 | X-tremeGENE HP DNA Transf. Reag. 0.4 ml 转染试剂 | 0.4 ml |
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| 4913850001 | FS Universal SYBR Green Master | 4 x 1.25 ml (for 200 reactions of 50 µl final reaction volume) |
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