磁珠蛋白A免疫沉淀试剂盒

磁珠蛋白A免疫沉淀试剂盒 10006D

描述

This kit provides a faster and easier solution for your immunoprecipitation experiments than Sepharose or Agarose, and includes all reagents required to perform small scale immunoprecipitation (IP) using your own antibody (Ab) of choice. The kit is optimal for standard IP, as well as co-IP and ChIP. The kit contains the widely used Dynabeads® Protein A and include all required buffers for immunoprecipitation.

When using this kit, you will:

• Reduce your protocol time to <30 minutes.
• Decrease or even eliminate background caused by non-specific binding—Easily remove of all supernatant after incubation and washing, with no risk of sample loss
• Minimize antibody usage and maximize target protein recovery—Rapid binding kinetics and high capacity
• Keep your protein complexes intact—Gentle magnetic separation and rapid binding kinetics avoid physical stress on your precious proteins
• Improve reproducibility—Consistent high performance of the Dynabeads®
• Increase confidence in results—Optimised and reproducible buffer compositions

Applications:
• immunoprecipitation (IP),
• chromatin immunoprecipitation (ChIP),
• small scale antibody purification and other protein isolations.

The magnetic separation technology is fast and gentle, with no centrifugation, rapid binding kinetics and short protocol time. Hence your precious target proteins will experience minimal physical stress. This permits the isolation and concentration of labile complexes that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and intact, large protein complexes will be preserved.

Benefits and Features:
• Superior signal-to-noise properties
• Highly reproducible results
• No columns, centrifugations, or time-consuming pre-treatment of your samples
• Gentle, minimal physical stress on precious proteins
• Easy handling and simple, short protocol.

The product holds reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.

免疫沉淀进入“磁性”阶段——Dynabeads®磁珠产品

货号名称目录价 10001DDynabeads® Protein A for Immunoprecipitation1 mL
177010003DDynabeads® Protein G for Immunoprecipitation1 mL
177010006DDynabeads® Protein A Immunoprecipitation Kit （40 reactions）4150
10007DDynabeads® Protein G Immunoprecipitation Kit （40 reactions）4150

货号名称目录价
10006DDynabeads® Protein A ImmunoprecipitationKit（40reactions）415010002DDynabeads® Protein A Immunoprecipitation（5mL）7260
10007DDynabeads® Protein G ImmunoprecipitationKit（40reactions）4150
10004DDynabeads® Protein G Immunoprecipitation （5mL）7260
10003DDynabeads® Protein G for Immunoprecipitation （1mL）1770
10001DDynabeads® Protein A for Immunoprecipitation（1mL）1770
12321DDynaMag™-2 Magnet（最佳工作体积：10–2,000 μl；可放置16个标准的1.5-2ml微量离心管，1个）7240
12321D（磁力架）DynaMag™-2 Magnet 1 each 7,240

描述

新型DynaMag™-2磁体已经过优化，可配合Dynabeads® 用于小体积样品的高效磁性分离。DynaMag™-2磁体具有强大的磁力，能够进行有效的分离，同时其采用了灵活且精巧的人体工学设计。上部的试管架可轻 松从基座内的磁体上取下，以便进行重悬、涡旋混合或样品手动振荡。试管架的中心轴可确保所有试管具有相同的涡旋混合效果。您还可以取下试管架，用于样品管 储存。该磁体可与所有类型的Dynabeads® 配合使用，用于蛋白质和核酸的高效分离。

磁架可以放置16个样品，实现多个样品的同步处理。磁架每侧有8个空位，您可以从96孔板反应中轻松扩大您的实验规模。

• 最佳工作体积：10–2,000 µl

• 可以放置16个标准的1.5 - 2 ml微量离心管

• 很好地控制和观察分离过程