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Co-IDA Agarose Beads 6FF,5ml

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  • 8.33/

江苏千株松生物科技有限公司
17302508337 00000000
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  • Brand  千株松
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Last Updated 2025-08-12 18:01

Co-IDA Agarose Beads 6FF,5ml

金属螯合亲和层析介质,又称固定金属离子亲和色谱,其原理是利用蛋白质表面的一些氨基酸,如组氨酸能与多种过渡金属离子如Cu2+,Zn2+,Ni2+,Co2+,Fe3+发生特殊的相互作用,能够吸附富含这类氨基酸的蛋白质,从而达到分离纯化的目的。因此,偶联这些金属离子的琼脂糖凝胶就能够选择性地分离出这些含有多个组氨酸的蛋白以及对金属离子有吸附作用的多肽、蛋白和核苷酸。一般来说,Ni2+是用于纯化组氨酸标记的蛋白质的优选金属离子。半胱氨酸和色氨酸也能与固定金属离子结合,但这种结合力要远小于组氨酸残基与金属离子的结合力。

镍亲和层析介质具有特异性好、流速快的优点,非常适合放大生产。

技术指标

产品名称

Co-IDA Agarose Beads 6FF

结构成分

6%交联琼脂糖

粒径

45-165μm


载量

50mg/ml

适宜PH值

3-12

操作温度

4-30℃

保存条件

4-8℃

应用:分离带His标签的重组蛋白及能被金属离子吸附的多肽、蛋白、核苷酸、磷酸化蛋白。

Company Name 江苏千株松生物科技有限公司
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