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EpiQuik Viral RNA Isolation Fast Kit
唾液RNA提取试剂盒
鼻拭子RNA提取试剂盒
鼻咽拭子RNA提取试剂盒
| Input Type: | Nasal Swab, Nasopharyngeal Swab, Saliva |
| Research Area: | Epidemiology |
| Target Application: | Sample Isolation |
| Vessel Format: | Columns/Tubes |
| 100% Guarantee: | 6 months |
The EpiQuik™ Viral RNA Isolation Fast Kit is a complete set of optimized buffers and reagents suitable for quick preparation of viral RNA from cell-free liquid specimens (excluding plasma and serum), specifically from saliva and nasal or nasopharyngeal swabs. The specialized buffering system allows RNA to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and pure RNA is eluted. The RNA purified with the kit can be used for a variety of routine applications, specifically for RT-PCR. This kit has the following advantages and features:
- Fast procedure delivers high-quality total RNA in total 10 minutes
- Ready-to-use RNA for high performance in downstream applications
- Consistent RNA yield from a small amount of starting material
Total RNA was isolated from nasal swab samples with the EpiQuik™ Viral RNA Isolation Fast Kit and used for RT-PCR analysis. A: Nasopharyngeal swab samples were spiked with SARS-CoV-2-N positive control; B: Nasopharyngeal swab samples were not spiked with SARS-CoV-2-N positive control. PCR was processed with use of primers and probes against SARS-CoV-2 N1/N2 and human RNase P gene (internal control).
Principle & Procedure
The kit contains all the reagents required for successfully performing RNA isolation directly from cell-free samples (excluding plasma and serum). After lysis, binding, and wash, RNA is easily recovered in quantities of up to 5 µg using specially designed columns. Total RNA is then ready to be used for a variety of downstream applications.
Starting Materials
The amount of starting materials can be up to 400 µl of liquid volume, with the best volume of 200 µl. A total of 50 standard extractions (using 200 µl of sample) can be performed with this kit.
Competitor Kit Comparison
| Kits | EpiQuik Viral RNA Isolation Fast Kit | Competitor Q Kit | Competitor Z Kit | Competitor O Kit |
|---|---|---|---|---|
| Sample Types | Cell-free biological fluids and liquid specimens (excluding plasma and serum) | |||
| Run Time | 10 minutes | 20-40 minutes | 10 minutes | 15-20 minutes |
| Yield | >95% recovery | >90% recovery | Unspecified in documentation | Unspecified in documentation |
| Price Per Preparation | $ | $$$ | $$ | $$ |
| Processing | Manual | Manual; Automation (requires vendor-specific equipment) | Manual | Manual |
| Sample Input Volume | 400 µl | 140 µl | 400 µl | 150 µl |
