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LT101S Omni-Easy™速溶型蛋白上样缓冲液(变性

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上海雅酶生物医药科技有限公司
18701893452 021-34781650
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  • Brand  雅酶货号LT101S
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Last Updated 2025-08-12 18:02

LT101S Omni-Easy™速溶型蛋白上样缓冲液(变性

Omni-Easy™速溶型蛋白上样缓冲液(变性,还原型,5×)
Omni-Easy™Protein Sample Loading Buffer(Denaturing,Reducing,5×)
本产品常温运输;保存于-20保质期12个月。

货号规格
goodLT101SL     1 mL×5
goodLT101SL     1 mL×15 
goodLT101LS     1 mL×75

产品特点
gd抑制降解——采用新型配方,确保煮样后pH值为中性,避免蛋白降解,尤其适用于磷酸化蛋白;

gd沉降迅速——样品会快速沉到加样孔底,不易飘起;

gd快速融化——从-20℃取出后融化速度快,不粘稠;

gd无异味——采用新型还原剂,不含β-巯基乙醇或DTT,无令人不快的臭味。

产品简介
good本品用于蛋白质SDS-PAGE电泳时的蛋白上样操作。其采用新型配方,确保煮样后pH值为中性,避免蛋白降解。在4℃时保持液体状态,从-20℃取出后急速融化,节省等待时间。采用新型还原剂,在保证强效还原性的同时,避免了β-巯基乙醇或DTT的臭味。

使用说明

good1. 使用时,在蛋白质样品中加入1/4体积本产品,混匀; good2. 将混合物用沸水浴加热5~10 min; good3. 加热结束后,高速离心5 min,上清即可用于电泳分析。如蛋白样品在加入上样缓冲液后,无不溶性残渣,则只需短暂离心,将混合液收集到管底即可。加热后,如果管盖上出现较多冷凝水,建议轻弹离心管将冷凝水和样品混合以后,再进行高速离心步骤,此举有助于降低上样样品浓度不均的现象。


注意事项

good1. 由于含有还原剂,使用本品上样的凝胶在银染时可能会发黄或出现条纹; good2. 由于含有变性剂,本品不适用于非变性胶的电泳; good3. 为了您的安全和健康,请穿实验服并戴一次性手套操作; good4. 本产品仅限科研使用。

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good9. ZHUANG, Qian, et al. Astragalus Polysaccharides Ameliorate Diet-Induced Gallstone Formation by Modulating Synthesis of Bile Acids and the Gut Microbiota. Frontiers in Pharmacology, 2021, 12.(IF 5.810)
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