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纯度> 85 % as determined by SDS-PAGE.内毒素< 1.0 EU per μg protein as determined by the LAL method.生物活性1. Immobilized human TNFRSF9 (Cat:10041-H08H) at 10μg/mL (100μL/well) can bind human TNFSF9 (Cat:15693-H01H),the EC50 of human TNFSF9 is 7-40ng/mL.
2. Measured by its ability to induce IL-8 secretion by HT1080 transfected with human CD137. The EC50 for this effect is typically 0.3-1.2 μg/mL.蛋白构建A DNA sequence encoding the human TNFSF9 (NP_003802.1) (Arg71-Glu254) was expressed with the Fc region of human IgG1 at the N-terminus.Accession#NP_003802.1表达宿主HEK293 Cells种属Human预测 N 端Glu分子量The recombinant human TNFSF9 consists 444 amino acids and predicts a molecular mass of 47.9 kDa.缓冲液Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
