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HIGHLIGHTS
- High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
- Unique column design: Zero buffer retention and low elution volume (10 µl)
DESCRIPTION
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
| • | For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion, etc. No messy precipitations! |
| • | Innovative colored buffers* for rapid identification of complete bacterial cell lysis and neutralization steps. |
| • | Unique column design: zero buffer retention and low-volume (≥10 μl) elution. |
