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FFPE RNA/DNA提取试剂盒

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  • 118.06/

上海莱枫生物科技有限公司
18012236082 021-64810180
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  • Brand  lifefeng
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  • Main Business:PCR,核酸纯化,核酸电泳
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Last Updated 2025-08-12 18:06

FFPE RNA/DNA提取试剂盒

FFPE RNA/DNA提取试剂盒不使用二甲苯等有机溶剂,可直接从石蜡包埋组织/切片中分别提取RNA和DNA。优化的裂解条件能在20 分钟左右分散厚度≤10μm的组织切片,约30分钟即可充分释放RNA(此时DNA处于未溶解状态),通过离心可实现RNA和DNA的分别提取,电泳观察获得的RNA可见明显的18S RNA和少量28S RNA。

后续提取过程可采用硅胶膜吸附过柱法或者磁珠法。磁珠法试剂预分装于96孔板,配套使用16/32/48通道核酸纯化仪,洗脱体积为100 μl,洗脱液为去离子水,仪器运行时间约15分钟。

获得的DNA可直接应用于PCR、NGS等后续实验,获得的RNA可直接应用于RT-PCR。

 

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Company Name 上海莱枫生物科技有限公司
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Business Scope PCR,核酸纯化,核酸电泳
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