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Agencourt AMPure XP -核酸纯化试剂盒

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贝克曼库尔特国际贸易(上海)有限公司
400-168-5267
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  • Brand  BECKMAN COULTER
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  • Main Business:离心机、流式细胞仪及试剂、实验室自动化工作站、多重基因表达定量分析系统、各类临床诊断仪器及试剂
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Last Updated 2025-08-12 18:14

Agencourt AMPure XP -核酸纯化试剂盒

Agencourt AMPure XP是一种能够获得高质量DNA产物的核酸纯化试剂盒;纯化后的核酸纯度高,无盐离子残留,无需离心或过滤;既可以手工操作,也可以使用自动化工作平台以96孔或384孔板的形式完成。
• 200bp以上的核酸回收率高达90%以上,核酸长度最小要求100bp
• 有效去除dNTP、引物、引物二聚体、盐离子和其它杂质
• 纯化后的产物在4℃温度下保持7天不被降解
• 双链和单链DNA都能得到纯化
• 长达18个月有效期
• 手工操作和自动化工作站操作皆可
该试剂盒应用于: PCR体系纯化、酶切和连接反应体系纯化

产物适合于下游应用:PCR、Genotyping(SNP detection)、sequencing(Sanger and Next Generation Sequencing)、Cloning、Primer Walking

纯化产物得率高
Agencourt AMPure XP可以结合大于100bp的核酸片段,对于片段长度为10KB的长片段,也能获得比传统过柱法高得多的得率。
图1:经过3种纯化试剂纯化后的产物得率对比:分别是200bp、800bp和10KB,纯化所用的试剂分别为Agencourt AMPure XP、QIAquick 和QuickStep 过柱法纯化试剂盒。

1. 加入AMPure XP 磁珠试剂
2. 磁珠与核酸结合
3. 结合有核酸的磁珠与杂质分离
4. 乙醇洗涤
5. 加入洗脱液
6. 转移洗脱产物
产品 试剂盒组成 货号
Agencourt AMPure XP 5 mL Kit Agencourt AMPure XP Reagent A63880
Agencourt AMPure XP 60 mL Kit Agencourt AMPure XP Reagent A63881
Agencourt AMPure XP 450 mL Kit Agencourt AMPure XP Reagent A63882

*本文涉及的内容与产品仅用于科研和工业,不用于临床诊断。
Company Name 贝克曼库尔特国际贸易(上海)有限公司
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Business Scope 离心机、流式细胞仪及试剂、实验室自动化工作站、多重基因表达定量分析系统、各类临床诊断仪器及试剂
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