Qubit™ dsDNA HS Assay Kit
描述
The Qubit dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. Which product to choose for dsDNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit dsDNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA HS Assay Kit with microplate reader
Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.
3. Also available as a "1X" dsDNA assay kit that provides reagent and buffer in a formulation that is stable as a ready-to-use solution for up to one year after preparation.
For Research Use Only. Not for use in diagnostic procedures.
图表
Performance of the Qubit® dsDNA HS AssayPerformance of the Qubit® dsDNA HS Assay. The Qubit® dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA." href="https://medicineplatform.com/file/upload/202508/12/181505711.jpg">
Performance of the Qubit® dsDNA HS Assay
Performance of the Qubit® dsDNA HS Assay. The Qubit® dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA." href="https://medicineplatform.com/file/upload/202508/12/181505711.jpg">Performance of the Qubit® dsDNA HS Assay
Three reconstituted lots of CHO CD EfficientFeedâ„¢ B AGT were compared to a lot of catalog liquid product. (Cat. No. A10240-01) in a reconstituted DG44 CHO fedbatch shake flask experiment in CD CHO Medium. A rDG44 CHO un-fed batch culture in CD CHO Medium was an additional control. The results indicate that the 3 lots of reconstituted AGT and the liquid control performed equivalently.
Increase in accuracy and precision using the Qubit®Accuracy and precision of the Qubit® Quantitation Platform. Ten replicates of lambda DNA at concentrations from 0.01 to 10 ng/µl were assayed using the Qubit® DNA HS Assay on the Qubit® 2.0 Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in 10 replicates using a NanoDrop® ND-1000 Spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are the concentrations of DNA in the starting samples, before dilution in the Qubit® assay tubes." href="https://medicineplatform.com/file/upload/202508/12/181505201.jpg">
Increase in accuracy and precision using the Qubit®
Accuracy and precision of the Qubit® Quantitation Platform. Ten replicates of lambda DNA at concentrations from 0.01 to 10 ng/µl were assayed using the Qubit® DNA HS Assay on the Qubit® 2.0 Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in 10 replicates using a NanoDrop® ND-1000 Spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are the concentrations of DNA in the starting samples, before dilution in the Qubit® assay tubes." href="https://medicineplatform.com/file/upload/202508/12/181505201.jpg">Increase in accuracy and precision using the Qubit®
规格
| Sample Type (General): | dsDNA |
|---|---|
| For Use With (Equipment): | Qubit® Fluorometer |
| Quantitation Range: | 0.2-100 ng |
| Number of Reactions: | 500 reactions |
| Product Line: | Quant-iT™, Qubit™ |
| Product Size: | 500 assays |
| Green Features: | Fewer resources used and less waste |
| Shipping Condition: | Room Temperature |
