Zymo Research货号D5031现货EZ DNA Methylation-Lightning Kit(规格包装200次/盒)(目录价¥8213.00元/盒)上海睿安生物/上海睿强生物13611631389
Zymo Research货号D5031现货DNA甲基化修饰试剂盒Lightning(EZ DNA Methy-lation-Lightning Kit)(规格包装200次/盒)(目录价¥8213.00元/盒)美国Zymo Research代理:上海睿安/睿强生物13611631389
Zymo Research生物公司位于美国加州Orange County，创建于上世纪90年代初，主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒，其DNA甲基化，羟甲基化，核酸提取等产品已得到世界各top-level实验室的认可，产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出，独占鳌头。产品已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络，并且销售额快速增长。使用其产品的论文已经在各种权威性科技期刊(如NATURE自然、SCINECE科学、PNAS美国科学院院刊、Stem Cells干细胞、 Nucleic Acids Res核酸研究等)上发表。
Highlights
- Fastest bisulfite conversion kit for complete bisulfite conversion of DNA for methylation analysis.
- Ready-to-use conversion reagent is added directly to DNA.
- High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
Product Description
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
Technical Specifications
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. |
|---|---|
| Conversion | >99.5% |
| Elution Volume | ≥10µl |
| Equipment | Thermocycler with heated lid and microcentrifuge. |
| Input | 100pg - 2µg of DNA. |
| Processing Time | 1.5hours |
| Recovery | >80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
