λDNA /HindⅢ Marke

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  • 48.61/

上海觅拓生物科技有限公司
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  • Brand  BM
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  • Main Business:细胞,载体,试剂,技术服务,实验室耗材
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Last Updated 2025-08-12 18:27

λDNA /HindⅢ Marke

浓    度:500ng/5μl

 

储运温度:4℃(长期保存请置于-20℃)

 

制品说明:

        λDNA /HindⅢ Marker为已含有1×loading buffer的DNA溶液,可取5μl直接电泳,使用方便,电泳图像清晰。

        λDNA /HindⅢ Marker由DNA片段23130bp 、9416bp、6557bp、4361bp、2322bp、2027bp、564bp以及125bp构成,无非特异带型,无DNA降解酶。

 

使用注意: 

1. 电泳时的加样孔宽度小于6mm时,每次取5μl 制品电泳便可得到清晰条带。如果加样孔增宽,须适当增加Marker制品的加样量。

2. 对DNA电泳而言,Agarose的纯度对DNA条带的清晰度影响很大。因此,电泳时应尽量选用质量好的Agarose。

3.Agarose电泳时,Agarose的浓度与DNA片段的分离性能关系密切。Agarose浓度越大,对短片段DNA分离性能越好;反之,Agarose浓度越小,越有利于长片段DNA的分离。

4.电泳时的电压不宜过高,尽量控制在5V/CM左右;电泳缓冲液尽量是新鲜配制,特别是在做标准图片时。

 

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Company Name 上海觅拓生物科技有限公司
Location
Business Scope 细胞,载体,试剂,技术服务,实验室耗材
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Medicine Platform
 
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