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Western封闭液

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  • 10.42/

北京百瑞极生物科技有限公司
010-82422342
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  • Brand  Biorigin
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Last Updated 2025-08-12 18:41

Western封闭液

别名:膜封闭液

规格:100 ml,500ml 

保存:2-8℃保存,有效期一个月。 

产品简介: 

     Western Blot 膜封闭液(Blocking Buffer)适用于 Western Blot 实验中 PVDF 膜或 NC 膜等转印膜的封闭。封闭后,可以减小后续的一抗或二抗和膜的非特异性结合,降低背景,增强信噪比,以达到理想的显色效果。按照每张膜封闭需要 5-10 毫升封闭液计算,一个包装的 Western 封闭液可以封闭 10-20 张膜。 

使用方法: 

     本产品为即用型,无需稀释。蛋白转膜后将转印膜置于容器中,加入足够量的膜封闭液充分覆盖膜,室温下 振荡封闭半小时至 1 小时,即完成膜的封闭,然后进行一抗孵育等后续操作。 

注意事项:

     通常在室温封闭 60 分钟即可。对于一些背景非常高的抗体,可以 4℃封闭过夜。为了您的安全和健康,请穿实验服并戴一次性手套操作。

Company Name 北京百瑞极生物科技有限公司
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