Ki67 /MKI67 蛋白, Human

Ki67/MKI67 is localized on mitotic chromosomes and maintains its dispersion during nuclear envelope disassembly. It covers a large portion of the chromosome surface and prevents chromatin from collapsing into clumps. Ki67/MKI67 Protein, Human (GST) is the recombinant human-derived Ki67/MKI67 protein, expressed by E. coli , with N-GST labeled tag.

Ki67/MKI67 蛋白对于维持核膜解体后单个有丝分裂染色体在细胞质中的分散至关重要。Ki67/MKI67 位于有丝分裂染色体的表面，特别是在染色体周围层内，覆盖了染色体表面的很大一部分，防止染色体塌陷成单个染色质团。作为具有高净电荷的表面活性剂，它建立了空间和静电荷屏障，促进独立的染色体运动。Ki67/MKI67 表现出 DNA 结合能力，显示出对超螺旋 DNA 和富含 AT 的 DNA 的偏好。虽然它对有丝分裂染色体的内部结构没有贡献，但它在染色质组织中的作用仍然不确定，这增加了这可能是其维持分散的有丝分裂染色体的主要功能的间接结果的可能性。该蛋白与各种伙伴相互作用，包括 KIF15、NIFK、PPP1CC，并形成涉及 ZNF335、HCFC1、CCAR2、EMSY、RBBP5、ASH2L 和 WDR5 的复合物的一部分，表明其参与复杂的细胞过程和分子网络。

Human

E. coli

N-GST

P46013-1 (M1-P120)

4288  [NCBI]

MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDIRIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESLQNGRKSTEFPRKIREQEP

approximately 36.91 kDa

• Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.