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合肥基赛生物供应Fermentas RevertAid-TM

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合肥基赛生物科技有限公司
13155125588 00000000
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Last Updated 2025-08-12 18:41

合肥基赛生物供应Fermentas RevertAid-TM

RevertAid™ First Strand cDNA Synthesis Kit是一个完整的以RNA为模板高效合成第一链cDNA的反应体系。该试剂盒可合成长达13 kb的cDNA。

该试剂盒使用的RevertAidReverse Transcriptase具有的RNase H 活性比AMV reverse transcriptase低。

试剂盒配套提供的重组RiboLock™ RNase Inhibitor有效保护RNA模板免于降解。该产品与逆转录反应完全兼容,因为其在50°C时仍然保持活性。

试剂盒配套提供oligo(dT)18和随机六聚体引物。oligo(dT)18与mRNA 的poly(A)尾选 择性结合。随机六聚体引物转录无需poly(A) 尾,因此可转录mRNA的5’-端区域,也可以无poly(A) 尾的RNA (micro RNAs)为模板合成cDNA。试剂盒也可使用基因特异性引物。

第一链cDNA可直接作为PCR (见RT-PCR操作说明书)、实时PCR或第二链cDNA 合成(见操作说明书)的模板。

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